摘要
目的研究交联透明质酸（HA）膜的特性与生物相容性。方法测定交联HA膜的膨胀率；用扫描电镜观察其表面结构；采用咔唑法测定其体外对透明质酸酶的抗酶解性；膜片植入大鼠皮下，观察其降解情况。结果交联HA膜的膨胀率为2．7～3．0，离子强度和pH值对膨胀率无影响；在电子扫描电镜下，吸水后的膜表面呈蜂窝状结构．切面呈片层状、膜管状结构，膜管开口呈长圆形，未被酶降解的干膜结构致密，而降解的膜内部结构疏松，膜表面有被分离的片状结构；体外酶降解实验表明酶的浓度与降解产物之间呈线性关系；膜的体内降解曲线显示植入物在体内的降解速度早期较快，10d后呈减慢趋势，40d后又呈回升状态，60d后降解接近80％。结论交联HA膜所具有的膨胀性、抗酶解性和较好的生物相容性，使这一新型生物材料用于药物载体的开发具有潜力。
Purpose To test the characteristics and biocompatibility of the film made of cross-linked hyaluronic acid（HA）. Methods Swelli these films were examined using scanni their resistance to enzymatic degradatio ng ratios of the films were measured and the surface morphologies of ng electron microscopy （SEM）. Carbazole method was used to measure n. The films were implanted subcutaneously in rats and the turnover of the films was examined. Results The average of swelling ratios was 2.7- 3.0. No significant effects of ionic strength and pH on the swelling ratio of the films were observed. Under SEM, the graphs showed the appearance of a honeycomb structure of the surface and a sheet-like and membranous tube-like structure of the crosssection of the films after hydration. The tube-like structure has a hexagonal or elliptic opening. A condensed structure with unbroken surface of dry films was observed. In contrast, the digested films showed a less condensed structure with some fragments dissociated from the surface. The result of in vitro degradation demonstrated a linear relationship between the concentration of enzyme and the products of degradation. The plot of in viva degradation of the films indicated that the turnover rate of the implant was quick in the earlier period, stepped down after 10 days,and again stepped up after 40 days. Approximately 80% of weight of the implant was digested after 60 days. Conelusion The films made of cross-linked HA were able to swell, resistant to enzymatic degradation and bioeompatible, thus allowing the new biornaterials to act as a potential drug delivery carrier.
出处
《中国生化药物杂志》
CAS
CSCD
2006年第1期25-28,共4页
Chinese Journal of Biochemical Pharmaceutics
关键词
交联透明质酸
膜
膨胀率
生物相容性
eross linked hyaluronic acid
film
swelling ratio
biocompatibility
