摘要
福莫司汀对培养的ＨＬ６０细胞ＤＮＡ的损伤寿江１，冯杏婉（北京药理毒理研究所，北京１００８５０，中国）１ＮｏｗｉｎＤｅｐａｒｔｍｅｎｔｏｆＴｏｘｉｃｏｌｏｇｙ，ＩｎｓｔｉｔｕｔｅｏｆＲａｄｉａｔｉｏｎＭｅｄｉｃｉｎｅ，Ｂｅｉｊｉｎｇ１００８５０，Ｃｈｉ...
IM: To detect DNA damage
caused by fotemustine (Fot). METHODS: DNA damage in HL60 cells was
evaluated by modified alkaline elution technique. DNA interstrand
crosslink (ISC) and DNA protein crosslink (DPC) were determined.
Carmustine (Car) was used as control. Drug treatment time was 1 h .
RESULTS: After treatment with Fot 300 μmol·L -1 , ISC and DPC
index were 5 4 and 6 1 at 6 h, 2 7 and 1 2 at 12 h,
respectively. ISC reached the maximum at about 6 h. For Fot 100 μ
mol·L -1 , ISC and DPC index at ISC peak time were 2 1±0 9
and 3 55±0 23, 5 0±0 5 and 7 7±1 1 for Car 100 μmol·L
-1 , respectively. Single strand break (SSB) was induced by Fot.
CONCLUSION: Fot caused HL60 cell DNA ISC, DPC, and SSB. ISC was
formed more quickly by Fot than that by Car.
出处
《中国药理学报》
CSCD
1996年第5期474-477,共4页
Acta Pharmacologica Sinica
关键词
福莫司汀
DNA
损伤
白血病
HL60
肿瘤细胞
fotemustine
cross linking reagents
DNA damage
leukemia
HL60
cultured tumor cells
