摘要
人早幼粒白血病ＨＬ６０细胞及其亚系中ｍｄｒ１基因和癌基因表达的关系周卫东，张鸿卿，方敏，薛绍白（北京师范大学生物系，北京１００８７５，中国）关键词癌基因；多种抗药性；流式细胞计量术；急性早幼粒白血病目的：研究人早幼粒白血病ＨＬ６０细胞系及其分别抗Ｈ...
IM: To study the relationship between expression of
oncogenes and multiple drug resistant (MDR) phenotype. METHODS: The
drug resistant level of HL60 cell line and its sublines were
determined with flow cytometry. RNA Dot blot hybridization was used
to identify the expression of oncogenes and mdr 1 gene. RESULTS:
The expression of mdr 1 gene was in the opposite relation with c
myc expression, but in the positive relation with c H ras
gene expression in the multiple drug resistant cell lines. In non
MDR cell line HL60/RA, the expression levels of mdr 1, c myc
, and c H ras were the same as HL60 parental cells. CONCLUSION:
Multiple drug resistance is related to not only mdr 1
expression, but also some oncogenes expression level.
出处
《中国药理学报》
CSCD
1996年第5期471-473,共3页
Acta Pharmacologica Sinica
关键词
癌基因
多种抗药性
白血病
HL60
表达
oncogenes
multiple drug resistance
flow cytometry
acute
promyelocytic leukemia
