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RAd-ODC/Ex3as对肺癌A-549细胞抑制作用的研究 被引量：1

Inhibition of lung cancer cell A-549 by rAd-ODC/Ex3as
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摘要 目的探讨重组腺病毒rAd-ODC/Ex3as对肺癌A-549细胞的体外抑制作用。方法利用基因GFP测定病毒感染效率,采用MTT法观察rAd-ODC/Ex3as对肺癌细胞A-549生长增殖的影响,用Western Blot检测rAd-ODC/Ex3as对鸟氨酸脱羧酶(ODC)基因表达的影响。结果当MOI为50时,腺病毒感染A-549细胞的效率约为75%;Western Blot证实rAd-ODC/Ex3as可抑制ODC基因的表达;rAd-ODC/Ex3as以20MOI感染肺癌A-549细胞可明显抑制其生长增殖。结论rAd-ODC/Ex3as在体外能有效地干扰ODC基因的表达,并可抑制肺癌A-549细胞的生长和增殖,有望成为治疗肺癌的基因药物。 Objective To study the effects of rAd-ODC/Ex3as on lung cancer ceils in vitro. Methods The infection rate of rAdODC/Ex3as was measured with the aid of GFP expression, Western Blot technique was used to observe the inhibition of ODC expression in infected tumor cells. The malignant phenotype of A-549 cells was assessed by growth curve. Results Approximate 75% of A-549 cells were infected with rAd-ODC/Ex3as when MOI reached 50. The expression of ODC was inhibited in the infected tumor ceils, rAd-ODC/Ex3as could inhibit A-549 cell growth and invasive ability at 20 of MOI. Conclusions rAd-ODC/Ex3as could inhibit effectively the expression of ODC gene and the growth of lung cancer cell A-549,It may be one of the promising medicines for ant;sense gene therapy in lung cancer.
作者 李林 田辉 刘贤锡 张岩 张冰
机构地区 山东大学齐鲁医院胸外科 山东大学医学院分子生物学实验中心
出处 《中国老年学杂志》 CAS CSCD 北大核心 2006年第1期83-84,共2页 Chinese Journal of Gerontology
基金 山东大学博士后基金资助项目(2005)
关键词 鸟氨酸脱羧酶 腺病毒载体 肺肿瘤 A-549细胞 基因治疗 Ornithine decarboxylase Adenovirus vector Lung neoplasms A-549 cells Gene therapy
分类号 Q78 [生物学—分子生物学] R737.2 [医药卫生—肿瘤]
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参考文献13

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二级参考文献7

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共引文献8

同被引文献10

  • 1卢圣栋.现代分子生物学实验技术[M](第2版)[M].北京:中国协和医科大学出版社,2001.191.
  • 2Carlisle DL,Devereux WL,Hacker A,et al.Growth status significantly affects the response of human lung cancer cells to antitumor polyamine-analogue exposure[J].Clin Cancer Res,2002,8(8):2684-2689.
  • 3Asher G,Bercovich Z,Tsvetkov P,et al.20S Proteasomal degradation of ornithine decarboxylase is regulated by NQO1[J].Mol Ccll,2005,17(5):645-655.
  • 4Cui XS,Kim NH.Polyamines inhibit apoptosis in porcine parthenotes developing in vitro[J].Mol Reprod Dev,2005,70(4):471-477.
  • 5Huang Y,Pledgie A,Caseroin RA Jr,et al.Molecular mechanisms of polyamine analogs in cancer cells[J].Anticancer Drugs,2005,16(3):229-241.
  • 6Choi KS,Suh YH,Kim WH,et al.Stable siRNA-mediated silencing of antizyme inhibitor:regulation of ornithine decarboxylase activity[J].Biochem Biophys Res Commun,2005,328(1):206-212.
  • 7Wallon UM,O' Brien.Polyamines modulate carcinogen-induced mutagenesis in vivo[J].Environ Mol Mutagen,2005,45(1):62-69.
  • 8Wolter F,Ulrich S,Stein J.Molecular mechanisms of the chemopreventive effects of resveratrol and its analogs in colorectal cancer:key role of polyamines?[J].J Nutr,2004,134(12):3 219-3 222.
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  • 10Belting M,Borsig L,Fuster MM,et al.Tumor attenuation by combined heparn sulfate and polyamine depletion[J].Proc Natl Acad Sci USA,2005,99(1):371-376.

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二级引证文献2

中国老年学杂志
2006年 第1期

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