摘要
目的了解炔咪菊酯的慢性经口毒性和致突变性。方法按GB15670-1995《农药登记毒理学试验方法》进行大鼠慢性经口毒性实验(6个月喂养),小鼠急性经口、骨髓多染红细胞微核试验和睾丸初级精母细胞染色体畸变试验,Am es试验。结果小鼠急性经口LD50:雌性为926 mg/kg,雄性为681 mg/kg;骨髓多染红细胞微核试验为各剂量组的微核率与阴性对照组微核率比较差异无显著性(P>0.05);睾丸初级精母细胞染色体畸变试验为各剂量组染色体畸变细胞率与阴性对照组比较差异无显著性(P>0.05);Am es试验为阴性;慢性经口毒性实验结束时高剂量组动物血清乳酸脱氢酶(LDH)和球蛋白(GLB)均降低,白/球蛋白比(A/G)升高,雄性动物体重降低,部分动物心、肝、脾、肺、肾、脑、睾丸有不同程度的病理表现,心体比、肝体比和卵巢体比均升高;中剂量组部分动物心、肝、脾、肺、肾、脑有不同程度的异常表现,雄性动物心体比升高。结论本受试物在本次试验所用剂量下无致突变作用;慢性毒性经口的最大无作用剂量44.05 mg.kg-1.d-1。
Objective To study the chronic oral toxicities and mutagenic effects of imiprothrin mother liquor. Methods The chronic oral toxicities in SD rats, acute oral, bone marrow micronucleus, chromosome aberration test in testicle spermatocytes in mice, Ames assay were conducted based on the national standardized testing method（GB15670 - 1995）. Results Acute oral LDs0 was 926 mg/kg in female mice and 681mg/kg in male mice. No significant differences（P 〉0.05）were observed in micronudeus rates and chromosome aberration rates between the controlled and all dose groups in bone marrow micronucleus test and chromosome aberration test. A negative result was observed in Ames assay at all dose groups, compared with the controlled. No obvious toxic response was observed in the treated animals during the chronic test. At the high dose group, LDH and GLB decreased while the albumin/globulin ratio increased, body weights decreased in the males. Histopathologlcal examinations revealed that various changes were found in the heart, Liver, spleen, lung, kidney, brain and testicle in the high dose group, as well as in the heart, liver, spleen, lung, kidney and brain in the medium dose group. It also found the heart/body, liver/body and oval/body weight ratio increased in the high dose group and heart/body weight ratio increased in male rats at the medium dose. There were no obvious pathological changes in the tested organs in the low dose group. Conclusion Imiprothrin mother liquor was shown as a non-mutagenic substance. The 180-day oral no-observed-adverse-effect level （NOAEL） of imiprothr/n in SD rats was 44.05 mg·kg^-1 · d^-1.
出处
《中国职业医学》
CAS
北大核心
2006年第1期32-35,共4页
China Occupational Medicine
