摘要
目的:了解细胞信号转导过程中,内化作用如何调节HGF-cM et的信号转导,Dynam in蛋白对HGF-cM et内化作用中的影响。方法:以标记的HGF刺激体外培养的HepG2细胞,并在一组细胞中转染无功能的突变Dynam in蛋白,进行细胞摄入的对照实验。结果:突变Dynam in蛋白在37℃时降低了HGF-cM et的内化率约63%,而4℃时内化量一直维持较低水平。结论:HGF所涉及的信号转导由其受体cM et蛋白所介导,与细胞的内吞过程紧密相连,Dynam in蛋白在内化过程中起重要作用。
Objective：To investigate the internalization how to regulate the signal transduction of HGF-cMet, specially the influence of Dynamin on internalization of HGF-cMet by receptor inducing. Methods：Hepatocyte growth factor was labeled with ^125Ⅰ. The quantity of HGF internalization of ^125Ⅰ HGF in HepG2 cell at 4℃ and 37℃ and different time point was compared respectively with cell uptake assay. Results： Internalization of ^125Ⅰ-HGF in HepG2 cell was reduced about 63% in cell line of K44A mutation Dynamin transfected at 37℃. At 4℃, the level of interlization maintained very low. Concluslon：The process of signal transduction was induced by HGF receptor cMET and was related with internalization of cell. The danamin is important role in process of internalization.
出处
《军医进修学院学报》
CAS
北大核心
2006年第1期1-3,共3页
Academic Journal of Pla Postgraduate Medical School
基金
国家自然科学基金资助项目(30370706)
