摘要
用OKT系列和Ia单克隆抗体和免疫荧光技未测定11例不能手术的晚期直肠癌病人周围血OKT_3,OKT_4,OKT_8和Ia阳性细胞白分率并与16例正常人作对照,比较冷冻治疗前后T细胞亚群Ia阻性细胞和血清免疫球蛋白的改变。冷冻术前患者周围血OKT_3和OKT_4阳性细胞明显减少(p<0.01);但OKT_8阳性细胞增加(P<0.01);OKT_4/OKT_8比值明显减少(P0.01)。这些结果提示晚期直肠癌病人免疫平衡明显失调,OKT_8阳性细胞增加可能与肿瘤分泌的肿瘤抗原特异牲抑制因子有关。冷冻治疗后,OKT_4和Ia阳性细胞明显增加,OKT_4/OKT_8比值虽没有恢复正常,与冷冻前比较,有所增加。提示冷冻治疗对宿主免疫平衡的调节起一定作甩。血清IgA ,IgM,IgG在冷冻治疗前后无显著差异,但与正常人比较:显著增加,其意义还不清楚。
By means of immunofluorescence and dual direction agar diffusion techniques, the subset of T lymphocytes, Ia positive cells and serum immunoglobulins have been detected in patients with rectal carcinoma and a comparison was made between pre-and post-cryotherapy. In the patients of pre-cryotherapy the percentage of OKT3 and OKT4 positive cells was significantly decreased as compared to that in control (0.01<P<0.05), but OKT8 positive cells markedly increased (P<0.01) and the ratio of OKT4/OKTs significantly decreased (P<0.01). These results suggested the presence of immunological imbalance in patients with advanced rectal cancer. In patients of post-cryotherapy, the percentage of la and OKT4 positive cells was significantly increased as compared to that in patients of pre-cryotherapy (P< 0.01). These showed that the cryotherapy might play a role in modulating immunological balance of tumor host. The level of serum IgA, IgG and IgM did not show significant difference before and after therapy, but serum IgG was markedly in-ceased in patients as compared to normal control. The significance of increased IgG deserves further investigation.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1990年第5期365-368,共4页
Chinese Journal of Cancer
