摘要
采用野生罗布麻(Apocynum venetamL.)顶芽及芽下茎段为外植体进行离体培养及快繁技术优化的研究。结果表明:适宜罗布麻离体培养的基本培养基为MS,适宜外植体起始分化的培养基为:MS+BA 1.8mg/L+KT 0.5mg/L,分化率为88.9%以上;适宜茎段增殖的培养基为MS+BA 2.0mg/L+KT 0.5mg/L,繁殖系数最高达5.67倍,通过切段繁殖还可提高繁殖系数3倍;适宜生根的培养基为MS+IBA 0.5mg/L+NAA 0.02mg/L,生根率达90%以上。
The techniques of tissue culture and rapid propagation were researched with terminal buds and stem segments below bud ot wild dogbanes （ Apocynum Veetam L. ） as explants. The results showed that the basic medium suitable for culturing dogbanes （ Apocynum venetam L. ） in vitro was MS. The medium suitable for starting the differentiation of explants was MS ＋ BA 1 .8 mg/L＋ KT 0.5 mg/L with the differentiation rate of over 88.9%. The medium suitable formultiplication of stems was MS ＋ BA 2.0mg/L ＋ KT 0. 5mg/L with the highest multiplication factor of 5.67 times, which could also increase 3 times by catting stem. The medium suitable for rooting was MS ＋ IBA 0.5mg/L ＋ NAA 0. 02mg/L with a rooting rate of over 90%.
出处
《生物技术》
CAS
CSCD
2006年第1期72-75,共4页
Biotechnology
基金
国家林业局"948"项目("罗布麻属耐盐植物新种及其培育技术引进
No.2004-4-10)
关键词
罗布麻
离体培养
快繁技术
dogbane （ Apocynum venetam）
culture in vitro
rapid propagation techniques
