摘要
目的:建立微波辐射实时生物效应的监测方法,初步探索实时条件下微波辐射对PM A刺激的大鼠腹腔巨噬细胞生物发光的影响。方法:将微波通过微带天线导入微弱生物化学发光仪的样品舱,实现微波辐射的实时生物效应监测。以大鼠腹腔巨噬细胞为研究对象,观察了样品在距微波天线不同距离及不同的微波调制脉宽、调制频率对巨噬细胞发光的影响。结果:样品距微波天线0.5cm时,一定条件的微波辐射可引起巨噬细胞生物发光的增强。当脉宽一定时,调制频率为1000、500PPS时,其生物发光的增强率为5.07%、2.17%;当调制频率为1000PPS时,脉宽为2000ns、1000ns时,其生物发光的增强率为10.17%、2.88%。结论:初步实现了监测微波辐射实时效应的方法,并观察到微波辐射实时作用下可使PM A刺激的巨噬细胞生物发光增强。
Objective： To establish the method of real-time monitoring the effect of microwave and observe the effect of microwave irradiation on the bioluminescence of PMA active rat＇s peritoneal macrophage. Method： Firstly, an equipment of real-time monitoring the biological effects of microwave irradiation was assembled. Microwave was modulated and amplified, and then the sample cabin of ultraweak chemiluminescence＇s set by a microstrip antenna was conducted. Secondly, we investigated the bioluminescence changes of macrophage under microwave irradiation. Results： When the distance between the sample and microwave antenna was 0.5 cm, the microwave irradiation could increase the bioluminescence of macrophage. As the pulse width was set to 2000 ns and modulation frequency was 1000, 500PPS, the rate of enhancement was 5.07%, 2.17% respectively. As the modulation frequency was set to 1000 PPS and pulse width was 2000, 1000 ns, the rate of enhancement was 10.17%, 2.88% respectively. Conclusions： The method of real time monitoring the effect of microwave was established initially and microwave irradiation could enhance the bioluminescence of PMA active macrophage in real time.
出处
《中国医学物理学杂志》
CSCD
2006年第1期28-30,共3页
Chinese Journal of Medical Physics
基金
国家自然科学基金资助项目(30370363)
关键词
实时监测
微波辐射
巨噬细胞
生物发光
real time monitor
microwave irradiation
macrophage
bioluminescence
