摘要
比较赤霉菌粗毒素液或外源SA预处理抗病和感病小麦幼苗不同时间,叶片活性氧代谢变化.结果表明:赤霉菌粗毒素液处理小麦幼苗根部2～48 h后,O2-产率先增加后降低,感病品种(川麦28)H2O2含量逐渐增加;用外源SA预处理小麦幼苗叶片2 h后再用赤霉菌粗毒素液处理小麦幼苗根部2～48 h,抗、感病品种的O2-产率和H2O2含量均明显提高,且抗病品种(89107)先于感病品种达到其峰值,粗毒素液处理24 h后抗性品种保护酶(SOD,CAT和POD)活性比感病品种的高.抗病品种PAL活性变化对SA预处理响应强烈.SA可能作为信号分子之一,诱发小麦幼苗对赤霉菌粗毒素液产生一定的系统抗性.
Changes in O2 production, H2O2 content and defensive enzyme （SOD, CAT, POD and PAL） activities in wheat （ Triticum aestivum） leaves treated with Gibberella sauhinetii toxin or with the toxin after a pretreatment with salicylic acid （SA） were studied. O2 production increased at first, followed by a decline, when the seedling roots were pretreated by immerging them in the toxin solution for 2 - 48 h, and H2O2 content in the disease - sensitive variety Chuanmai 28 increased gradually. On the other hand, in the treatment of immerging the seedling roots in the toxin solution for 2 -48 h after a pretreatment with SA by immerging the seedling leaves in an SA solution for 2 h, O2 production and H2O2 content in the disease- resistant variety 89107 elevated and reached its maximum much more quickly than those in Chuanmai 28, and SOD, CAT and POD activities in 89107 were higher than those in Chuanmai 28. Phenylalanine ammonia lyase （ PAL） activity in 89107 rose rapidly, suggesting that PAL activity of disease - resistant wheat varieties reacts strongly to SA pretreatment. It is therefore postulated that SA may be a signal, which could regulate active oxygen species （ AOS） metabolism and induce systemic acquired resistance to the toxin.
出处
《西南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第6期851-854,876,共5页
Journal of Southwest Agricultural University
关键词
小麦
赤霉菌粗毒素
活性氧
水杨酸
Gibberella saubinetii toxin
active oxygen species （AOS）
defensive enzyme
salicylic acid （SA）
