摘要
目的了解雌激素和克罗米酚对海人藻酸(KA)致癎大鼠癫癎发作行为学的影响及其影响癫癎活动的部分机制。方法将去势的雌性大鼠添加雌激素(20mg/kg)或添加雌激素和克罗米酚治疗,比较各组大鼠致癎后癫癎发作的行为学变化;并采用间接免疫荧光法检测各组大鼠海马中γ-氨基丁酸(GABA)免疫反应细胞及GABAA受体α1亚单位表达的变化。结果添加雌激素治疗组大鼠癫癎发作的潜伏期和到达4/5级(4级或5级)的时间[分别为(24.63±11.44)min和(41.50±16.22)min]均较去势组[分别为(46.75±14.61)min和(65.13±12.99)min]明显缩短,而同时添加雌激素和克罗米酚治疗组的潜伏期[(43.50±5.75)min]比单纯添加雌激素组明显延长。雌激素组的阳性免疫反应细胞数在大鼠海马的某些区域也较去势组明显减少,克罗米酚组与雌激素组相比则有所增多。结论高水平的雌激素可促进癫癎发作,克罗米酚添加治疗具有一定的抗癫癎作用。这可能与脑内GABA能系统某些功能的改变有关。
Objectives To find out the effects of estrogen and clomiphene on behavior of epileptic rats induced by kainic acid （KA） and probe into some mechanisms. Methods Ovariectomized Sprague- Dawley female rats were treated with estrogen （E） or estrogen and clomiphene （ C ）. Their behaviors when they were induced seizures were observed and compared. Indirect immunofluorescence method was used to measure the alterations of gamma-aminobutyric acid （GABA） immunoreactive cells and α1 subunits of GABAA receptors in the hippocampus of all groups. Results The latency and time at reaching 4/5 degrees in KA＋E group （（24.63 ± 11.44） minutes and （41.50 ± 16.22） minutes, respectively） were reduced greatly than KA group （ （46.75 ± 14. 61 ） minutes and （65.13 ± 12.99） minutes）, while the latency of KA ＋ E ＋ C group （ adding estrogen and clomiphene, （ 43. 50 ± 5. 75 ） minutes） became prolonged signitlcanfly than in KA ＋ E group. Conclusion High-level estrogen should be proconvulsant and the clomiphene might have some antiepileptic effects, which may be related with some alterations of GABA energic function in the brain.
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2005年第12期778-781,共4页
Chinese Journal of Neurology
