摘要
目的:探讨低氧运动对大鼠血清促红细胞生成素(EPO)浓度及其在肾脏中EPO mRNA基因表达的影响,为高原训练方案的制定提供理论依据。方法:采用ELISA和RT-PCR技术分别测定大鼠血清EPO浓度和肾脏EPO mRNA表达水平。结果:低氧应激后,大鼠血清EPO均显著性升高(p<0.05),大鼠肾脏EPO mRNA表达虽呈上升趋势,但只有急性低氧安静组、急性低氧运动组和急性低氧训练组有显著性升高(p<0.05),而间歇低氧安静组和间歇低氧训练组变化不明显。结论:低氧应激,血清EPO和肾脏EPO mRNA表达升高是机体自我保护机制之一,低氧训练能使血清E-PO升高,可能是低氧环境和运动缺氧共同刺激的结果;而低氧训练后EPO mRNA表达水平变化不明显,可能是4000m海拔高度缺氧的刺激较强烈,对后续的表达造成反馈抑制作用的原因。
Objective ： To study the effects of serum erythropoietin and the expression of EPO of kidney on rat during hypoxia training, the theory of project of high altitude training is provided. Methods ： Serum EPO and the expression of EPO mRNA of kidney in rat by EISA and RT- PCR technique were determined respectively. Results： Serum EPO of rat increased more significantly（p 〈 0.05） by hypoxia stress, the expression of EPO mRNA of kidney in rat increased, but they increased more significantly（p 〈 0.05）only in Acute hypoxia control group, Acute hypoxia exercise group and Acute hypoxia training group, they increased little in intermittent hypoxia control group and intermittent hypoxia training group. Conclusion： It is one of the mechanism of protection that serum EPO and the expression of EPO mRNA of kidney increase by hypoxia stress , it is possible that serum EPO increase after hypoxia training by effects of hypoxic environment and exercise hypoxia; it is possible that is more intense stress of hypoxia in altitude 4000m to produce feedback - control effects finally , so that the expression of EPO mRNA changed a little .
出处
《广州体育学院学报》
北大核心
2006年第1期44-47,共4页
Journal of Guangzhou Sport University
