摘要
基于自组装技术在玻碳电极表面构筑了肌红蛋白-纳米氧化铝模板-金胶复合组装体,研究了该纳米复合组装体的电化学性质。循环伏安和电化学交流阻抗实验结果表明,该纳米组装体结构致密稳定,固定在组装体中的肌红蛋白在0.2 mol/L醋酸-醋酸钠缓冲液中于-0.21 V(vs.Ag/AgC l)附近有一对准可逆的氧化还原峰,为肌红蛋白血红素辅基Fe(Ⅲ)/Fe(Ⅱ)的特征峰。带负电荷的金纳米粒子能为蛋白质分子提供具有生物兼容性的微环境,从而有利于其电子传递反应的发生。
Nano-structured system of myoglobin-alumina template-colloidal gold on the glassy carbon electrode surface has been successfully fabricated by assembly technology. The electrochemical properties of myoglobinnano-alumina templates-colloid gold assembly system were investigated. The adsorbed myoglobin displayed a pair of redox peaks in 0.2 mol/L NaAc-HAc buffer （ pH = 5.4） with a formal potential of - 0. 21 V （ vs. Ag/AgCl）, being the characteristic peaks of the myoglobin heme Fe （ Ⅲ ） / Fe （ Ⅱ ） redox couples. The colloid gold nanoparticles of negative charges provide a microenvironment similar to that of the redox protein in native systems and give the protein molecules more freedom in orientation, thus reduce the insulting property of the protein shell for the direct electron transfer and facilitate the electron transfer.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2006年第1期80-82,共3页
Chinese Journal of Analytical Chemistry
基金
国家科技部"863"计划资助(No.2002AA233051)
关键词
肌红蛋白
纳米氧化铝模板
金胶
电化学性质
Myoglobin, alumina template, colloidal gold, electrochemical properties
