摘要
目的研究不同手法针刺对外周血单核细胞(PBMC)细胞信号转导子和转录活化子(STAT5)mRNA和DNA的结合能力的影响。方法将30例健康受试者分补法针刺组(补足三里)、泻法针刺组(泻足三里)、正常对照组各10例。采集外周血经Ficoll-Hypaque梯度离心获得PBMC,用逆转录-聚合酶链反应(RT-PCR)进行STAT5mRNA半定量检测;凝胶阻滞电泳(EM-SA)分析PBMC中STAT5与DNA结合活性。结果补法针刺足三里穴可显著提高人PBMC的STAT5mRNA基础转录水平和STAT5与特定DNA的结合能力(P<0·01),但泻法针刺足三里穴的试验结果与健康人正常水平无显著性差异(P>0·05)。结论补法针刺免疫调节作用有细胞因子及JAK/STAT信号转导途径的参与,但影响STAT5mRNA转录水平及DNA结合的原因并不明了。
Objective To study on effects of different manipulation methods Of acupuncture on the binding ability of signal transducers and activators of transcription （STATS） in human peripheral blood mononuelear cells （PBMC） with DNA. Methods Thirty healthy volunteers Were randomly divided into 3 groups; reinforcing method group, reducing method group, normal control group, 10 eases in each group, The expression of STATS mRNA and the activation of STATS in the human PBMC were detected by reverse transeriptase-polymerase chain reaction （RTPCR） and electrophoretie mobility shift assay （EMSA）. Results In the reinforcing method group, the basic transcription level of STAT5 mRNA in human PBMC and the binding ability of STATS with DNA significantly increased （P〈0.01）, but in the reducing method group, they did not significantly change as compared with those in the normal control group （P〉0.0S）, Conclusion Cytokines and JAK/STAT signal transduction pathway are involved in immunoregulative actions of acupuncture of the reinforcing method, but the reasons of influencing the transcription level of STATS mRNA and the binding ability of STATS with DNA are unclear.
出处
《中国针灸》
CAS
CSCD
北大核心
2006年第2期120-122,共3页
Chinese Acupuncture & Moxibustion
