摘要
用逆转录－ＤＮＡ聚合酶链式反应（ＲＴ－ＰＣＲ）从１例中国庚型肝炎（ＨＧＶ）患者血清中扩增出含Ｅ１前区部分基因及Ｅ１区５＇端共３０９ｈｐ基因片段。对其中包括Ｅ１区９６ｂｐ在内的１２０ｂｐ核苷酸进行了序列分析，发现此区段基因与录入号为Ｕ４４４０２Ｇｅｎｅｂａｎｋ报道的美国发现的ＨＧＶ相关序列具有９３％的同源性，氨基酸的同源性为９８％。Ｇｏｌｄｋｅｙ蛋白质分析程序显示，此区段的Ｅ１区可能存在抗原位点。化学合成可能为抗原位点长约２９个氨基酸的多肽Ｅ１Ｐ１。利用Ｅ１Ｐ１及ＮＳ３区内短肽ＮＳ３Ｐ１包被的ＥＬＩＳＡ方法检测了１２名非甲－戊肝炎患者血清。Ｅ１Ｐ１检出的２份血清（２／１２）中有抗－Ｅ１Ｐ１ＩｇＧ的存在。ＮＳ３Ｐ１捡出的３份血清（３／１２）中有抗－ＮＳ３Ｐ１ＩｇＧ的存在，其中包括Ｅ１Ｐ１检出的两份阳性血清。本结果说明在ＨＧＶＥ１区内至少有一个抗原位点存在。
Gene fragments of partial putative pre-E1 and partial putative E1 region were amplified successfully from a serum of one patient with hepatitis G virus(HGV) infection by reverse iranscription-polymerase chain reaction (RT-PCR). A 120-nuceotide sequence in it was analysed by computer program. It was found that two parts of the nucleotide sequences derived from HGV in chinese and in USA reported by genebank were 93% identical, whereas an alignment of the amino acid sequences encoded by the two regions showed a 98% identity. The amino acid sequences encoded by the 120-nucleotide sequence were analysed by Goldkey program, the results showed that the region may have a potential antigenic epitope. The predicted epitope was synthetized chemically, a panel of non-A-E hepatitis human sera was tested with the synthetic peptides by enzyme-linked immunosorbent assay for epitope-specific responses. Of 12 non-A-E hepatitis serum samples, two samples (17%) demonstrated a spectrum of reactivity to the peptide.Moreover the two sera were also reactive to the synthetic peptide in HGV NS region. Results from this study suggest that the protein of HGV encoded by 5' E region contains at least one potential antigenic epitope.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
1996年第3期12-16,共5页
Chinese Journal of Cellular and Molecular Immunology
