摘要
目的探讨糖尿病患者红细胞膜ＡＴＰａｓｅ活性的变化。方法１７例胰岛素依赖型（ＩＤＤＭ）和４８例非胰岛素依赖型糖尿病（ＮＩＤＤＭ）患者红细胞膜Ｎａ~＋－Ｋ~＋－ＡＴＰａｓｅ、Ｃａ^（２＋）－ＡＴＰａｓｅ活性分别用改良的Ｈａｎａｈａｎ法和Ｌｕｔｈｒａ法测定，并设相应年龄对照组。统计方法用ｔ检验和直线相关分析。结果ＮＩＤＤＭ患者红细胞膜Ｎａ~＋－Ｋ~＋－ＡＴＰａｓｅ、Ｃａ^（２＋）－ＡＴＰａｓｅ活性分别下降了２４．３３％和１７．１１％，ＮＩＤＤＭ患者则分别下降２１．２４％和１６．４２％。两组糖尿病患者ＡＴＰａｓｅ活性均与糖化血红蛋白呈负相关，与年龄、空腹血糖或胰岛素水平无相关。结论糖尿病患者均有红细胞膜Ｎａ~＋－Ｋ~＋－ＡＴＰａｓｅ、Ｃａ^（２＋）－ＡＴＰａｓｅ活性下降，其原因与慢性高血糖有关。
The study was to discuss the changes of erythrocyte membrane ATPase activities.Methods Erythrocyte membrane Na+-K+-ATPase and Ca2+-ATPase activities were determined by modified Hanahan's and Luthra's methods tespectively(CV 7.2%and 3.8%,respectively)in17 IDDM and 48 NIDDM patients,compared with correspondent controls.t test and linear relationship assay were used as statistical method.Results Erythrocyte 5.membrane Na+-K+-ATPase and Ca2+-ATPase activities decreased 24.33%and 17.ll%in IDDM patients respectively,2l.24%and l6.42%in NIDDM patients respectively.Two ATPase activities vvere negatively correlated with HbA1c and were not correlated with the age, free blood glucose or serum insulin in IDDM and NIDDM patients,Conclusion Erythrocyte membrane Na+-K+-ATPase and Ca2+-ATPase activities were decreased in both IDDM and NIDDM patients and were probably due to chronic hyperglycemia.
