摘要
背景:骨形态发生蛋白-2是高效骨诱导因子。骨形态发生蛋白-2诱导骨内、外膜和骨髓基质细胞分化为骨母细胞,有加强骨床再生能力的作用,还可以活化或诱导血管周围的间充质细胞分化为软骨和成骨细胞。目的:观察益骨胶囊预防和治疗用药对去卵巢骨质疏松大鼠骨组织骨形态发生蛋白-2蛋白表达的影响。设计:随机分组设计、对照实验。单位:暨南大学附属第一医院中医科。材料:实验于 2003-03/10在暨南大学中药学实验室完成。动物:选择10月龄普通级雌性 SD 大鼠 72只,体质量(380±20)g,由广东省医学实验动物中心提供(合格证号:粤检证字第 2002A024号)。随机抽取24只分为假手术(腹部切口但不切除卵巢)预防用药组(12只)和治疗用药组(12只),余 48只行腹部切口双侧卵巢切除术复制骨质疏松模型后随机分为 4组:模型预防用药组、模型治疗用药组、预防用药组、治疗用药组,每组 12只。药物:益骨胶囊由淫羊藿、枸杞子、熟地黄、牛膝等组成,深圳三九医药股份有限公司提供免煎颗粒,配制成水溶液,含生药 1.0g/m L。干预:益骨胶囊预防用药于手术后 3d 即开始灌胃,假手术预防用药组、模型预防用药组灌胃生理盐水 3m L、预防用药组灌胃益骨胶囊水溶液 3m L,1次 /d,共 24周;益骨胶囊治疗用药于手术后第 13周开始灌胃,假手术治疗用药组、模型治疗用药组灌胃生理盐水 3m L、治疗用药组灌胃益骨胶囊水溶液 3m L,1次 /d,共 12周。免疫组化方法检测骨组织骨形态发生蛋白-2蛋白表达。主要观察指标:骨组织骨形态发生蛋白-2阳性反应细胞的平均灰度值。结果:大鼠造模 24周时,模型预防用药组、预防用药组、假手术预防用药组大鼠骨组织骨形态发生蛋白-2阳性反应细胞的平均灰度值分别为81.84±10.01,124.46±6.55,130.15±10.09,模型预防用药组明显低于假手术预防用药组 (P <0.01),预防用药组显著高于模型预防用药组(P<0.01),与假手术预防用药组相近(P >0.05)。模型治疗用药组、治疗用药组、假手术治疗用药组大鼠骨组织骨形态发生蛋白-2阳性反应细胞的平均灰度值分别为 77.97±10.35,110.32±8.69,131.79±12.10,模型治疗用药组明显低于假手术治疗用药组(P <0.01),治疗用药组显著高于模型治疗用药组(P <0.01),与假手术治疗用药组相近(P >0.05)。结论:益骨胶囊预防和治疗用药均能促进骨组织骨形态发生蛋白-2蛋白表达。
BACKGROUND： Bone morphogenetic protein-2 is a high-efficient bone- inducing factor, which can induce the inside and outside periosteum and marrow-derived stromal cells differentiate into osteoblast, strengthen the regeneration of bone mattress, activate r lead MSCs around blood vessel to differentiate into chondroblast and osteoblast.
OBJECTIVE： To observe the effects of yigu capsule to be used for prevention and treatment on the expression of bone morphogenetic protein-2 in bone tissue of the ovafiectomized rats with osteoporesis.
DESIGN： A completely randomized grouping design and randomized control trial. SETTING： Department of Traditional Chinese Medicine, First Affiliated Hospital, Jinan University.
MATERIALS： The experiment was carried out in the laboratory of Traditional Chinese Medicine of Jinan University form March to October 2003. Animals： 72 normal grand female SD rats, with 10-month-old ages, weighted （380-20） g, provided by the center of laboratory animal science of Guaugdang （the quality exequatur number of No. 2002A024）, were adopted. Totally 24 rats were randomly chosen from them anti divided into preventive granp （12 rats） and therapeutic group （12 rats） of sham operation （only opened the rats＇ abdomen but not removed their ovary）, and the left 48 rats were randomly divided into 4 groups after replicating OP model by ovariectomy （which opened the rats＇ abdomen and removed their ovary）： OP model control group of prevention, OP model control group of treatment, OP model group of prevention with yigu capsule and OP model group of treatment with yigu capsule, with 12 rats in each group. Medicine： Yigu capsule was made up by epimedium, fruit of lyclum barbaruml, radix angelica sincnsis, root of aehyranthes bidentata blume etc, the decoct-free granule of the drugs were provided by Sherrzhen Sanjiu Medical ＆ Pharmaceutical Co., Lid, the aqueous solution was made to include crude drug 1.0 g/mL.
INTERVENTIONS： The prevented groups of yigu capsule were administered by gastric infusion 3 days ＇after ovariectomy, 3 mL NS was given to preventive group of sham operation and OP model control group and 3 mL aqueous solution of yigu capsule was given to preventive group with yigu capsule, 1 time a day for 24 weeks; the therapeutic groups of yiga capsule were administered by gastric infusion in the 13^th week after ovariectomy, 3 mL NS was given to therapeutic group of sham operation and OP model control group and 3 mL aqueous solution of yiga capsule was given to therapeutic group with yigu capsule, 1 time a day for 12 weeks, lmmunohistochemistry Was used to detect the expression of BMP-2 on protein level in bone tissue：
MAIN OUTCOME MEASURES： The mean gray （MG） of the BMP-2 positive reaction cells in bone tissue. RESLUTS： In the 24^th week after ovafiectomy, the MG of BMP-2 positive reaction cells in bone tissue of preventive OP model control group, preventive group with yigu capsule and preventive group of sham operation were 81.84±10.01, 124.46±6.55, 130.15±10.09, that of preventive OP model control group was obviously lower than that of preventive group of sham operation （P 〈 0.01）, and that of preventive group with yigu capsule was obviously higher than that of preventive OP model control group （P 〈 0.01） and closed to that of preventive group of sham operation （P 〉 0.05）. The MG of BMP-2 positive reaction ceils in bone tissue of therapeutic OP modal control group, therapeutic group with yigu capsule and therapeutic group of sham operation were 77.97±10.35, 110.32±8.69, 131.79±12.10, that of therapeutic OP model control group was obviously lower than that of therapeutic group of sham operation （P〈0.01）, and that of therapeutic group with yigu capsule was obviously higher than that of therapeutic OP model control group （P〈0.01） and closed to that of therapeutic group of sham operation （P〉0.05）.
CONCLUSION： The preventive and therapeutic treatment of yigu capsule could both improve the expression of BMP-2 in bone tissue on albumen level.
出处
《中国临床康复》
CSCD
北大核心
2006年第3期190-192,F0003,共4页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金(30472274)
广东省自然科学基金重点项目(04105837)
国家中医药管理局中医药科学研究专项(04-05JL19)~~
