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鸡白细胞介素18成熟蛋白基因真核表达质粒的构建及其在鸡胚成纤维细胞中的表达 被引量：8

Construction of Eukaryotic Expressing Plasmid of Chicken Interleukin 18 Mature Protein Gene and Its Expression in Chick Embryonic Fibroblasts(CEF)
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摘要 通过PCR方法自含鸡白细胞介素18(Chicken interleukin-18,ChIL-18)成熟蛋白(mature ChIL-18,mChIL-18)基因的克隆质粒中扩增mChIL-18基因,并将其克隆到真核表达载体pcDNA3.1/V5-His-TOPO中。阳性克隆鉴定后,在脂质体作用下转染鸡胚成纤维细胞(CEF),通过间接免疫荧光试验(IFA)检测到了mChIL-18在CEF中的表达。 Chicken interleukin 18 mature protein gene was amplified from the recombinant plasmid named pT-mChIL18 by polymerase chain reaction（PCR） and cloned into pcDNA3.1/V5 His TO PO. The recombinant plasmid was transfected into chicken embryonic fibroblasts（CEF） with li posome. 24,48,72 hours after transfection, the expression of mChIL-18 were successfully tested by indirect immunofluorescence assay（IFA） with the antiserum of mChIL-18 expressed in E. coli.
作者 胡敬东 赵宏坤 崔治中
机构地区 山东农业大学动物科学技术学院
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2006年第1期91-94,共4页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词 mChIL-18 转染 IFA mChIL-18 transfection IFA
分类号 S852.4 [农业科学—基础兽医学] Q786 [生物学—分子生物学]
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参考文献9

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  • 4Schneider K, Puehler F, Baeubrle D,et al. cDNA cloning of biologically active chicken interleukin-18[J].Journal of Interferon and Cytokine Research, 2000,20:879-883.
  • 5程相朝,赵德明,吴庭才,李银聚,张春杰.鸡IL-18真核表达载体的构建及其对新城疫疫苗免疫增强作用的研究[J].畜牧兽医学报,2005,36(5):476-481. 被引量：20
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二级参考文献28

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共引文献32

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畜牧兽医学报
2006年 第1期

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