摘要
用3S Spin DNA Agarose Gel Purification Kit试剂盒将与水稻苯达松敏感致死基因相连锁的RAPD遗传标记S20-420和S316-600回收纯化,连接于pGEM-T载体并克隆测序,得到了S20-420和S316-600的全序列,其长度分别为423 bp6、06 bp.将两端序列设计特异PCR扩增引物可用于检测水稻苯达松敏感致死基因和标记辅助育种.
Randomly amplified polymorphic DNA（RAPD） was employed to detect molecular markers linked to the bentazon susceptible lethality gene in rice. RAPD marker S20 - 420 and S316 - 600 were linked to the bentazon susceptible lethality gene in rice. S20 - 420 and S316 - 600 fragment were reclaimed by 3S Spin DNA Agarose Gel Purification Kit and cloned in T - easy vector. Sequencing finally showed that the S20 - 420 fragment was actually 423 bp in lenth, the S316 - 600 fragment was actually 606 bp. It is suggested that the sequence of RAPD markers might be used as a basis for synthesizing the specific PCR primers and for detecting the bentazon susceptible lethality gene in rice and molecular marker assisted selection（MAS）.
出处
《韶关学院学报》
2005年第12期78-81,共4页
Journal of Shaoguan University
基金
广东省自然科学基金资助项目(001446)
