摘要
目的探讨姜黄素(curcum in)抑制人结肠癌(Lovo)细胞增生的作用及其机制。方法采用细胞生长曲线、集落形成实验和噻唑蓝还原法(MTT)观察姜黄素抑制Lovo细胞增生的作用;采用流式细胞术(FCM)测定姜黄素对细胞周期的影响;采用吖啶橙荧光染色、透射电镜观察和DNA琼脂糖凝胶电泳观察姜黄素诱导细胞凋亡作用。结果姜黄素对Lovo细胞体外生长具有抑制作用,并具有剂量效应关系。姜黄素具有抑制细胞集落形成和细胞毒作用。FCM结果认为,姜黄素主要使细胞大量堆积于S,G2/M期,使细胞不能进入下一个周期。细胞荧光染色和超微结构观察姜黄素处理后的细胞均呈凋亡特征性改变,DNA凝胶电泳也显示姜黄素处理后的细胞DNA呈特征性凋亡梯形带。结论姜黄素可抑制人结肠腺癌Lovo细胞的增生。其机制可能是阻滞细胞周期的S,G2/M期,使细胞不能进入下一个周期,以及诱导细胞凋亡。
Objective It has been reported that curcumin （diferuloylmethane） could inhibit growth of several malignant cells both in vitro and in vivo. However, the mechanism of action is unknown. In this study, we investigated the inhibitory effects of carcumin on human colon carcinoma cell （Lovo） proliferation and its mechanism of action in vitro. Methods The effects of curcumin on Lovo cell growth was by means of growth assay, colony formation assay and MTr assay, cell cycle analysis was by means of flow cytometer（FCM） and apoptosis analysis was by means of acridine-orange staining, electron microscopy and DNA agarose gel electrophoresis. Results Curcumin inhibited cell growth in vitro in a dose-dependent manner. The ability of colony formation of Lovo cells treated with curcumin was depressed. MTT test showed that eurcumin was cytotoxic to cells. FCM showed that Lovo cells treated with curcumin were largely accumulated in S, G2/M phase which prevented cells from entering the next cell cycle. The characteristic morphological changes of apoptotic cell induced by curcumin was confirmed by acridine-orange staining and electron microscopy, the characteristic DNA ladders induced by curcumin was confirmed by DNA agarose gel electrophoresis. Conclusion Curcumin can inhibit Lovo cells proliferation and the cellular mechanism responsible for the action is to arrest the cell cycle in S2/M phase and to induce the apoptotic cell death.
出处
《肿瘤研究与临床》
CAS
2006年第1期4-7,共4页
Cancer Research and Clinic
关键词
姜黄素
结肠癌
细胞凋亡
Colon carcinoma
Curcumin
Apoptosis
