摘要
用HRP逆行示踪法,对成年大白鼠两侧坐骨神经端端吻合术后,再生轴突可塑性作了研究。术后1—12月不同时间内,在吻合端左侧0.8cm处,再横断坐骨神经,放入HRP,存活2天,取材观察。结果表明:所有动物脊髓腰骶段两侧前角均出现HRP标记细胞。标记细胞数量随吻合术后时间增长而增加。左侧前角较右侧前角标记细胞多。说明受损的坐骨神经轴突能再生,各自进入对侧的坐骨神经,向脊髓方向延伸。但是,仅部分再生轴突能延伸过缝合处的组织痂。本实验提示再生轴突的可塑性,它受环境因素的影响。
The plasticity of regeneration of sciatic nerve (NS) axons was studied by end-end anastomosing the bilateral sciatic nerves in adult rats with the retrograde horseradish peroxidase (HRP) tracing method. After a survival time varying from 1 to 12 month, the SN anastomosis was transected at a point 0.8 cm to the left of the original suture and the HRP was introduced to the cut end of the two nerve stumps 2 days before sacrifice. The results showed that HRP-labeled cells were located in the ventral horns on both sides of the lumbar and upper sacral spinal cord in all animals studied. The number of cells labeled increased rapidly with the survival time after formation of the anastomosis was increased, The numbers in the left sides of ventral horns remained high as compared to that observed on the right side of the spinal cords where relatively few labeled cells were encountered. these results suggest that injured SN axons can respectively regenerate into the contralateral SN graft and extended toward the spinal cord. However only some of the regenerated axons can actually elongate beyond the tissue scar at region of the suture. The experimental observation mptied the plasticity of regenerated axons which was influenced by circumferential factors.
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
1989年第4期26-31,共6页
Journal of Jinan University(Natural Science & Medicine Edition)
关键词
白鼠
坐骨细胞
轴突
再生
酶
Sciatic nerve, Axon, Regeneration, Ventral horn of spinal cord, HRP-labeled cell
