摘要
短路电流技术是研究上皮组织膜离子转运的一种方法。当膜两侧浸浴液具有相同的成分时,消除跨膜电位差所需要的电流便等于被非共轭力所驱动的离子电流的代数和;此电流即为短路电流。本文目的是描述短路电流技术的细节和设计改进的上皮细胞膜灌流装置。并通过氨氯吡咪和去甲肾上腺素影响上皮细胞膜短路电流的实验,介绍测定短路电流的一般方法。实验表明氨氯吡咪加入到粘膜侧引起短路电流下降,膜电阻增加(电导减小),去甲肾上腺素加到浆膜侧则引起短路电流增加,膜电阻减小(电导增加)。
The short-circuit technique is a method used in the study of ion transport by cpithelial membrane. If a membrane is bathed on both surfaces by a solution of identical composition, the current necessity to abolish the transmembrane electrical potential difference is equal to the algebraic sum of the ions that are driven by nonconjugate forces; this current is referred to as the short-circuit current (Isc). It is the purpose of this article to describe in detail this technique and to design a new circulation reservoir and support stand. The authors investigated the effects of amiloride and noradrenaline on short-circuit current and conduction using the short-circuit techniqtie. The results show that the addifion of amiloride to the outer surface bathing solution can decrease the Isc and Ct, whereby resistance is increased; and the addition of noradrenaline to the inner surface bathing solution can increase the Isc and Gt, whereby resistance is decreased.
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
1989年第2期23-31,共9页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
广东省自然科学基金
关键词
短路电流
上皮细胞膜
离子转运
Short-circuit current, Epithelial membrane, Ion transport
