摘要
目的研究神经型一氧化氮合酶(nNOS)在肾盂输尿管连接部梗阻(UPJO)中的表达及意义。方法病例组36例,均为非管腔外压迫所致的UPJO狭窄段标本,且镜下具有UPJO基本特征:管壁肌层平滑肌稀疏,肌束变细,其间纤维结缔组织堆积。对照组20例为肾肿瘤、肾盂输尿管连接部(UPJ)切缘无肿瘤细胞浸润的UPJ标本。采用免疫组化SABC法对2组UPJ组织进行nNOS染色,并用图像分析进行定量分析比较。结果nNOS主要表达于UPJ肌层平滑肌细胞胞质。UP-JO组与对照组肌层表达的平均吸光度A值分别为0.208±0.014和0.230±0.016,2组比较差异有统计学意义(P<0.01)。结论UPJO中nNOS表达减少,提示NO合成减少可能参与UPJO发病过程。
Objective To study the expression and significance of neuronal nitric oxide synthase （nNOS） in ureteropelvic junction obstruction （UPJO）. Methods Specimens of the narrow segment of the ureteropelvic junction （UPJ） were obtained from 36 eases of UPJO （without aberrant vessels, adhesive band compression and high insertion of ureteral junction）. Furthermore, the light microscopic examination demonstrated that smooth muscle fascicles were sparse and thin,and fibrous connective tissues between them were deposited. Control UPJ specimens were taken from 20 cases of renal neoplasm, and tissues were confirmed histologically to be unaffected. Immunohistoehemical staining （SABC method） was performed to detect the expression of nNOS in specimens of the 2 groups. Quantitative analysis was made using image analysis technique. Results Immunoreactivity to nNOS was predominantly present in the smooth muscle cell cytoplasm of UPJ. The mean density of nNOS in UPJO and control groups was 0. 208 ± 0. 014 and 0. 230 ± 0.016 ,respectively, which were significantly different between the 2 groups （ P 〈 0.01 ）. Conclusions The decreased expression of nNOS in UPJO suggests that the reduction of NO production may participate in the pathogenesis of UPJO.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2006年第1期20-21,共2页
Chinese Journal of Urology
