摘要
目的 建立D-半乳糖与Aβ25～35诱导的拟痴呆（Alzheimer’s diaease，AD）模型小鼠，观察绿茶多酚对其学习记忆活动和AchE活性的影响．方法 皮下注射D-半乳糖（120mg／kg，连续给药12周），并在第7周时，侧脑室注射Aβ25～35（4nmol／L），建立AD模型小鼠．利用跳台法和水迷宫方法评价绿茶多酚对AD模型小鼠学习记忆障碍的改善作用；并测定血液和脑组织中AchE的活性．结果 绿茶多酚明显延长模型小鼠跳台潜伏期，减少跳台错误次数；缩短游出迷宫时间和减少进入盲端的错误次数，而对脑组织和血浆AchE活性无明显影响．结论 绿茶多酚对D-半乳糖与Aβ25～35诱导的AD模型小鼠学习记忆障碍具有改善作用，其作用可能与脑内AchE活性无关．
Objective To study the effects of green tea polyphenol（GTP） on the learning and AchE activity of mice with Alzheimer＇s Disease induced by D-galactose and Aβ25～35. Methods D-galactose（120 mg/kg） was intraperitoneally injected into mice for 12 weeks and Aβ25～35 （4 nmol） was injected by icv to establish Alzheimer＇s Disease mice model. The protective and therapeutic effects of GTP were determined by using water maze test and step down test, and the Ache activity was detected. Results GTP ameliorated the deleterious effects of D-galactose and Aβ25～35, and thereby improved the animal＇s learning memory, prolonged latency time, and the error numbers were significantly reduced, but the AchE activity did not have any significant change. Conclusion GTP can improve learning behaviour of mice with Alzheimer＇s Disease induced by D-galactose and Aβ25～35 These effects don＇t concern the role of reducing the activity of Ache in brain tissues.
出处
《北华大学学报（自然科学版）》
CAS
2006年第1期47-50,共4页
Journal of Beihua University（Natural Science）
