摘要
目的探讨PM2.5及其组分对原代培养大鼠心肌细胞的毒性及其剂量-反应关系。方法对出生24h的SD大鼠的乳鼠分离心肌细胞进行原代培养,以不同浓度(0.1、1.0、10.0、20.0、30.0、100.0、200.0μg/ml)的PM2.5全颗粒物及其水溶性提取物、有机提取物染毒24h,采用MTT法测定细胞的存活率,并观察染毒对心肌细胞搏动的影响。结果0.1、1.0、10.0μg/ml的全颗粒物、水溶性提取物和有机提取物染毒时,心肌细胞存活率均随染毒浓度升高而上升,在10.0μg/ml时达到最高,随后,随浓度的升高而降低;有机提取物的毒性明显高于水溶性提取物,心肌细胞的搏动频率随染毒浓度的升高而降低。结论以细胞存活率为指标,观察到PM2.5及其组分对心肌细胞的hormesis效应;PM2.5及其组分还可抑制心肌细胞的搏动频率并存在剂量-反应关系。
Objective To understand the ambient PM2.5 component and the toxicity to primary cultured myocardial cells of neonatal rats. Methods Primary myocardial cells cultures were prepared from 1-day-oM Sprague-Dawley rats and treated with PM2.5 suspension and its organic and water-soluble extracts at various concentrations for 24 h. The cellular viability was measured with MTT methods, and the beat of myocardial cells was observed and counted under inverted microscope. Results PM2.5 suspension and its organic and water-soluble extracts increased the viability of myocardial ceils at the concentrations bolow 10.0μg/ml, but above this, they could significantly decrease the viability of myocardial cells with a dose-dependent manner. The toxicity of organic extract of PM2.5 was significantly higher than that of water-soluble extract. PM2.5 suspension and its organic and water- soluble extracts also dose-dependently inhibited the beat frequencies of myocardial cells. Conclusion PM2.5 and its extracts show a hormesis effect on the cultured myocardial cells in the case of the cellular viability is taken as the response parameter. PM2.5 and its extracts can inhibit the beat of myocardial cells.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2006年第1期17-20,共4页
Journal of Environment and Health
基金
国家自然科学基金资助项目(30230310
30571534)
关键词
空气污染
颗粒物
心肌细胞
Air pollution
Particulate matter
Myocardial cell
