摘要
目的探讨不同抗原修复方法对免疫组化染色结果的影响。方法将10种不同的单克隆抗体在7组不同抗原修复条件下,在同一实验条件、同一时间内运用EliVision免疫组化法进行实验。结果ER、PR、CD3、Bcl-2用pH9.0Tris-EDTA热修复表达效果好;C-erbB-2、PSA、CD117用10mmol/LpH6.0柠檬酸盐缓冲液热修复效果好;CK用0.125%胰蛋白酶修复表达效果好;CD34修复后表达过强;ER、PR、CD3、Bcl-2、C-erbB-2、PSA、CD117用水浴热修复的表达效果均优于微波热修复法。结论免疫组化的染色质量受不同抗原修复方法的影响。掌握抗体最佳的修复方法能极大提高免疫组化结果的阳性率及准确率。
Objective To study the effect of varies antigen repairing methods on the results of immunohistochemistry staining, Methods With Elivision immunohistoehemlstry method, experiments were conducted on ten deferent kinds of monoclonal aptibody under the condition of seven deferent antigen repairing, and same experimental environment and time. Results Satisfied expre,ssion effects were shown when ER, PR, CD3, Bcl - 2 in pH9.0Tris - EDTA heat repairing, C - erbB - 2, PSA. CD117 in citrate buffer heat repairing, and CK in 0. 125 96 of trypsin repairing solution. After being repaired, CD34 was over expressed.The expression effects of ER, PR, CD3, Bcl - 2, C - erbB - 2, PSA CD117 in water heat repairing method were superior to those in microwave heat repairing method. Conclusion The quality of immnunohistochemistry staining is affected by deferent antigen repairing method as well as the pH d repairing solutions. It can significantly improve the positive rate and accuracy of immnunohistochmistry staining results to master the best antibody repairing method.
出处
《宁夏医学杂志》
CAS
2006年第1期50-51,共2页
Ningxia Medical Journal
关键词
免疫组化
抗原修复
Immunohistochemistry staining
Antigen repairing
