摘要
建立了反相高效液相色谱法测定枇杷露中乌索酸的含量，同时采用光电二极管阵列检测器检测乌索酸色谱峰的纯度。色谱柱为Nova—Pak C18（3．9×150mm，4μm），流动相为甲醇-水（88：12），流速1．0mL／min，检测波长210nm，柱温25℃。乌索酸在0．0395～0．237μg范围内线性关系良好（r=0．9991），检出限为0．2mg／L（3a），平均回收率为98．7％。该法适于对含有乌索酸的药物进行质量评价。
A determination method of ursolic acid in Zhike Pipa Lu by reversed - phase high performance liquid chromtograph was established, At the time the purity of ursolic acid peak was detected by means of Photodiode Array Detector （PAD）. The chromatographic column, Nova- Pak C18 （3.9×150 mm, 4μm） , methanol -water （88： 12, V/V） mobile phase with 1.0 mL/min flow rate, the detected wavelength （210 nm） ,and the column temperature（25℃） were adopted. The calibration curve of ursolic acid was linear under the content of 0. 039 5-0.237μg. The correlation coefficient was over 0.999 1. The detected limit was 0.2 mg/L （3σ）. The average recovery of ursolic acid acid was 98.7 %. The method can be adapted to evaluate the quality of medicine that contains ursolic acid.
出处
《贵州师范大学学报（自然科学版）》
CAS
2006年第1期52-54,112,共4页
Journal of Guizhou Normal University：Natural Sciences
基金
国家"863"计划重点资助项目(2002AA2Z3217)
