摘要
目的探讨姜黄素对Hep-2(人喉癌细胞株)细胞诱导分化机制,为喉癌治疗提供理论依据。方法应用MTT(塞唑兰)比色法和流式细胞仪检测Hep-2细胞增殖抑制率及细胞周期各时相的变化和凋亡率,并通过电子显微镜观察经姜黄素诱导分化后的亚细胞结构改变。结果姜黄素对Hep-2细胞增殖有明显的抑制作用,且呈剂量依赖性,流式细胞仪分析,G1期细胞增多,S期细胞减少,C2/M期细胞相对增多,亚细胞结构、细胞空化,染色质趋边凝集。结论姜黄素能够抑制Hep-2细胞增殖,阻止G1期细胞向S期的进程,促进Hep-2细胞凋亡。
Objective To explore mechanism of eurcumin inducing Hep-2 cell differentiation, for laryngeal carcinoma supply theory. Methods MTT assay and flow cytomctry were applied to detect the proliferation suppressing rate of Hep-2 cell and the change and apoptosis rate of cell cycle phases. Using electron microscope to observe the change of sub-eytoarchitecture induced and differentiated by curcumin. Results Cureumin played a visible suppressing role in Hep-2 cell proliferation, and had dose-dependent character. By flow cytometry analyse, cells increased in G1 phase, cells decreased in S phase, and cells increased correspondingly in G2/M phase. We observed cell cavitation and ehromatin agglutination. Conclusion Cureumin can suppress Hep-2 cell proliferation, hold back cell course from GI to S, and accelerate Hep-2 cell apoptosis.
出处
《中国实验诊断学》
2006年第1期45-46,共2页
Chinese Journal of Laboratory Diagnosis
