摘要
目的观察不同恶性程度的胶质瘤细胞中RNA编辑酶ADAR2(RED1),ADAR3(RED2)mRNA水平的表达。方法对原代培养的正常人脑胶质细胞和胶质瘤细胞SHG-44,U-251,BT-325,应用ADAR2,ADAR3全长序列合成引物及合成的特异引物,用逆转录PCR及图像分析法检测ADAR2,ADAR3 mRNA水平的表达。ADAR基因表达水平用基因/β-肌动蛋白(-βactin)灰度比值表示。结果ADAR2在正常人脑胶质细胞中表达极弱,在低恶性度的胶质瘤SHG-44细胞中呈弱表达,在高恶性度的胶质瘤U-251,BT-325细胞中明显表达。ADAR3在正常人脑胶质细胞及苯乙酸处理前后的胶质瘤细胞中,均未见表达。结论ADAR2 mRNA水平高表达,可能与高恶性度胶质瘤的发生有关。
Objective To observe the expression of RNA editing deaminase ADAR2（RED1） ,ADAR3（RED2）mRNA on various degree malignant glioma cell lines. Methods Glial cells of human brain tissue were primarily cultured and glioma SHG-44, U-251, BT-325 cells were cultured in vitro. The expression of ADAR2 and ADAR3 mRNA were detected by semiquantitative reverse transcription-polymerase chain reaction （RT-PCR） and Image Analysis. The level of ADAR gene expression was expressed as ratio expression rate （RER） of ADAR gene/β-actin according to Computer Image analysis.Results We found that the expression of ADAR2 was observed in a very low expression level in brain glial cells and a low expression level in low - grade malignant glioma SHG-44 cells, a high expression level in high - grade malignant glioma U-251, BT-325 cells. We found that ADAR3 had no expression in brain glial cells and glioma SHG-44, U-251, BT-325 cells. Conclusion The enhanced expression of ADAR2 mRNA tonight be involved in high degree malignant glioma orgin.
出处
《中国实验诊断学》
2006年第1期23-26,共4页
Chinese Journal of Laboratory Diagnosis
基金
国家自然科学基金项目资助(批准号:30271334)
中国博士后科学基金资助项目(2003034028)
吉林省科学技术厅科学基金资助项目(200506161)
