摘要
从发霉植酸钠溶液中分离到一株产植酸酶的变灰青霉（Ｐｅｎｉｃｉｌｌｉｕｍｃａｎｅｓｃｅｎｓ）Ｐ４。以麸皮：玉米面：黄豆饼粉＝７：２：１为主要培养基成份，用优选法确定最适培养基为在上述基本培养基中添加４％（ＮＨ４）２ＳＯ４，１％葡萄糖，１．５倍水，自然ｐＨ。发酵过程的动态分析表明，该菌在上述条件下２８℃恒温培养６ｄ后植酸降解率可达９０％；无机磷含量由０．１３％增至０．５７％；可溶性蛋白含量由３．８０％增至７．６０％。用４％ＣａＣｌ２·２Ｈ２Ｏ水溶液抽提固体发酵物，植酸酶活性可达３１２Ｕ／ｇ干曲。
A phytase-producing strain separated from the mildewed solution of sodium phytate was studied. The basic medium of solid state fermentation (SSF) is wheat bran: corn meal: beau cake powder = 7: 2: 1. The optimal medium from optimization should be added 1% glucose and 4%(NH4) 2SO4 to the basic medium as well as the moisture is 150%. It shows from the dynamic data of SSFthat the degradation rate of phytate could be 90% at 28℃ after 6-d incubation, inorganic phosphorus and soluble protein are increased from 0. 13% to 0.57% and from 3.80% to 7.60% respectively. Meanwhile, the activity of phytase is 3. 12u / g(DW) when SSF culture is extracted with 4% CaC12.2HZO.
出处
《微生物学通报》
CAS
CSCD
北大核心
1996年第4期217-220,共4页
Microbiology China
关键词
变灰青霉
固态发酵
植酸
降解
Penicillium canescens, Solid state fermentation, Phytate degradation, Phytase
