摘要
用高度多态性探针ｇＦＭ３１对５９个谷子（ＳｅｔａｒｉａｉｔａｒｌｉｃａＢｅａｕｖ．）品种（包括地方品种、育成品种及品系）进行ＤＮＡ指纹分析，共鉴别出５８种类型，而黑谷和黑粒１５１６给出完全相同的带型，二者有可能是同一材料。通过对ｇＦＭ３１ＤＮＡ序列分析，未发现其中有小卫星ＤＮＡ和微卫星ＤＮＡ序列。该探针虽在谷子品种中显示很高的多态性，但与禾谷类的其他物种的ＤＮＡ杂交信号微弱，表现出较强的谷子基因组特异性。
Fifty-nine genotypes(landraces,varieties or breeder's lines)of foxtail millet(Setaria italica Beauv.)were used in DNA fingerprinting with a highly polymorphic probe, xPSF 31. Fifty-eight types were distingushed,with only two genotypes giving identical band patterns.DNA sequencing of xPSF31 did not reveal any relationship with mini-or micro-satellite sequences.The probe revealed only weak signals in genomic DNA from other cereal species,wheat,barley,maize,rice,and pearl millet,indicating that xPSF31 is a Setaria genus specific sequence.
基金
洛克菲勒基金
关键词
谷子
品种鉴别
指纹分析
Setaria italica,RFLP,Cultivar Identification,Fingerprinting
