摘要
采用聚合酶链反应（ＰＣＲ）技术对１００例肺炎患儿的鼻咽分泌物标本进行肺炎支原体ＤＮＡ（ＭＰＤＮＡ）检测，用以诊断ＭＰ感染。结果ＭＰＤＮＡ阳性２４例，其中婴幼儿１３例，３０例健康对照均阴性，整个实验过程可在２～３ｈ完成。ＭＰ阳性的２４例中血清冷凝集试验＞１：３２者仅１７例（７０．８％）。提示ＭＰ系小儿肺炎常见病原，婴幼儿支原体肺炎并不少见。
his peper reports the use of the polymerase chain reaction to detect the mycoplasma pneumoniae DNA(MPDNA)to diagnose the infection of MP. Samples of nasopharyngeal secrations from 100 children with pneumoiawere colleeted for detection.The results showed that MP DNA was positive in 24 of the 100 cases,of tha 24, 13 wereinfants.MP DNA was negative in the control group of 30children. The proceddures of the PCR can be finished within3 hurs. Among 24 positive MP DNA samples,only 17(70.8%)were positive when detected with the cold aggluti-nation test(cAT)(tite>1:32)。It is suggest that MP is the common pathogen which causes children pneumonia,and is not rare to be found in infants.The diagnosis with PCR are more rapid,sensitive and specific compared withCAT,Finally,we also discusses the clinical differences between the older and younger childien potients who sofferfrom mycoplasma pneumonia.
出处
《重庆医科大学学报》
CAS
CSCD
1996年第1期29-31,共3页
Journal of Chongqing Medical University
关键词
聚合酶链反应
肺炎支原体
儿童
Polymerase chain reaction
Mycoplasma pneumoniae,Serological cold agglutination test
