摘要
本研究采用小鼠神经母细胞瘤细胞（ＮＢＡ２）无血清培养建立神经细胞实验研究模型。以氨基酸自动分析术观察神经降压素（ＮＴ）对实验性神经细胞内氨基酸类神经递质的影响。结果发现：ＮＴ可使细胞内天门冬氨酸（Ａｓｐ）和谷氨酸（Ｇｌｕ）这两种兴奋性氨基酸含量显著降低（Ｐ＜０．０１），而对抑制性氨基酸中的甘氨酸（Ｇｌｙ）和γ－氨基丁酸（ＧＡＢＡ）的影响不大（Ｐ＜０．０５）。结果提示：ＮＴ使兴奋性氨基酸Ａｓｐ和Ｇｌｕ含量降低的作用可能是其延缓实验性神经细胞老化的机制之一。
erum-free culture of mouse neuroblastoma A2(NBA2)cells was used in the experimen-tal study of neurons. High speed amino acid analyzer was used to observe the effects of neu-rotensin(NT)and on amino acid neurotransmitters contents in experimental neurons. After addition of NT separately into the culture medium,the following results were ob-tained:NT caused a decrease in the levels of aspartic(Asp)and glutamic(Glu)acids(p<0. 01),with no significant influence on GABA and glycin. The results suggested that theseneuropeptides may play an important role in the modulation of Asp and Glu levels in cellsduring experimental aging.
出处
《上海医学》
CAS
CSCD
北大核心
1996年第3期132-134,共3页
Shanghai Medical Journal
基金
上海高等院校科学发展基金和河北省自然科学基金
关键词
神经降压素
神经细胞
天门冬氨酸
谷氨酸
NT
experimental neuron
Asp
Glu
high speed amino acid analyzer.
