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幽门螺杆菌中国菌株IS605插入靶基因的获得及分析 被引量：1

The insertion analysis of IS605 in Helicobacter pylori from China
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摘要 目的获得幽门螺杆菌插入因子IS605的插入靶位点并分析其插入特征。方法采用酶切、Southernblot、LA PCR体外克隆、连接、测序的方法获得不同菌株IS605的靶基因及拷贝数,并对插入位点进行分析。结果中国菌株G40A、G61B各两个拷贝的IS605分别插入在幽门螺杆菌功能未明的基因中;IS605插入位点的左侧(ORFA侧)富含“AT”。结论IS605的插入位点具有区域特异性的倾向性;LAPCR体外克隆法是研究原核生物已知基因的周边基因及其拷贝数的较好方法。 Objective To get the target gene of IS605 in Helicobacter pylori G4OA, G61B isolated in China and to analyze the sequence character of insertion site. Methods Southern blot analysis, LA PCR cloning and sequence comparison. Results The target genes of IS605 in G40A and G61B are unknown genes in Helicobacter pylori; the insertion sites of ORFA are rich in ＂AT＂. Conclusion IS605 in Helicobacter priori may have insertion sequence tropism.
作者 张茂俊 张建中
机构地区 中国疾病预防控制中心传染病预防控制所
出处 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 2005年第11期869-872,共4页 Chinese Journal of Microbiology and Immunology
基金 国家自然科学基金资助(NSFC39870032)
关键词 幽门螺杆菌 LA PCR IS605 插入位点 Helicobacter pylori LA PCR IS605 Insertion site
分类号 R378 [医药卫生—病原生物学]
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参考文献16

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二级参考文献11

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共引文献3

同被引文献12

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中华微生物学和免疫学杂志
2005年 第11期

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