摘要
目的小鼠经皮下SARS-CoVS DNA疫苗免疫后,研究其特异性T细胞及相关细胞因子的特性。方法SARS-CoVS DNA疫苗免疫BALBPc小鼠后,获取淋巴细胞悬液。经S抗原多肽刺激后,采用ELISA检测细胞培养上清液中IFN-γ的水平,利用流式细胞仪在单个细胞水平上检测IFN-γ和IL-2的表达及其关系。结果当S混合多肽刺激后,DNA疫苗免疫小鼠的淋巴细胞产生大量的IFN-γ,与对照鼠相比差异有统计学意义(P<0.01)。细胞亚群分析的结果表明,IFN-γ+和IL-2+的CD4+T细胞百分率明显高于CD8+T细胞。单独产生IL-2的细胞占大多数,其次为IFN-γ和IL22双阳性细胞,只产生IFN-γ的细胞很少。结论SARS-CoVS DNA疫苗免疫小鼠后可以诱导抗原特异性CD4+和CD8+T细胞的产生。
Objective To elucidate the characterization of antigen-specific T cells from mice after immunization with SARS-CoV S DNA vaccine. Methods BALB/c mice were immunized subcutaneously with SARS-CoV S DNA vaccine. By day 7 after vaccination, single cells from lymph nodes, spleen and lung were prepared and incubated with pooled SARS-S pepfides. The level of IFN-γ in the culture supematants was assayed by ELISA. The frequency of IFN-γ and IL-2-producing cells were determined at a single cell level with FACS. Results After stimulation with SARS-CoV S ppeptidees, significantly higher level of IFN-γ in culture supernatants from spleen and lung were observed in the group of SARS-CoV S DNA immunized mice than in those of control ones（ P〈0.01 ）. Further analysis indicated that most of cells expressed IL-2 alone, some cells produced both IL-2 and IFN-γ, and very few cells were IFN-γ^＋ alone. In addition, higher percentage of IFN-γ^＋ and IL-2^＋ T cells were detected in lymph nodes and spleens than in lungs. Conclusion Vaccination of mice with SARS-CoV S DNA induces in the generation of antigen-specific CD4^＋/CD8^＋ T cells, which may plays an important role in the control and prevention of SAILS infection.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2005年第11期940-943,共4页
Chinese Journal of Microbiology and Immunology
基金
国家重点基础研究发展计划资助项目(2001CB510007)
国家自然科学基金资助项目(30340012)
广州市科技计划科技攻关引导项目(2003Z3-E0491)
广东省教育厅联合赞助科技攻关项目资助
