摘要
目的建立一种新的大鼠肝脏热缺血再灌注模型。方法将大鼠肝脏的尾状叶、右侧叶、方叶和乳突叶切除后,用三个动脉夹分别夹住肝脏左叶和中叶的肝蒂,实现全肝的热缺血。实验中与肝叶切除未热缺血的对照进行对照,研究了全肝缺血后门腔血管间的分流情况,并分别观察了肝脏热缺血60、90、120min后大鼠的生存率以及肝脏功能指标的变化。结果肝脏部分切除总共耗时23±3min,没有肝脏出血,而且没有出现大鼠死亡;在全肝热缺血后能够观察到通过肝脏尾状叶所建立起来的门腔分流,缺血90min后,大鼠的存活率为60%。结论这种模型完全能够实现大鼠的全肝热缺血再灌注,操作简单,不必另行建立门腔分流,为临床动物实验中全肝热缺血的研究提供了基础。
Objective To establish a new warm ischemia model, which combining partial hepatectomy with clamping the remnant liver. Methods Used three arterial clip clamps hold the pedicles of the median and left lateral lobe respectively at the level of the hilum to achieve total warm ischemia after the resection of the caudate, right lateral, quadrate lobes, and papillary lobe. Used liver lobes were removal,but without warm ischemia rats as control group,portacaval intervessel shunt condition was observed after total liver ischemia simultaneously observation on survival rate of rats and changes of liver function marker at 60, 90, and 120 minutes respectively after warm ischemia. Result All rats survived after the liver partial resection,the removel procedure took 23±3 minutes, bleeding of resected liver was no occurred. By using liver candate lobe established portacaval shunts was observed after total liver warm ischemia. Rats survival rate was 60%. Conclusion The model at technical is much simple and can realize whole warm ischemia reperfusion and there is no need for extra-portalsystemie shunt. It can be a basic modal for the research of whose liver warm ischemia-reperfusion.
出处
《临床小儿外科杂志》
CAS
2005年第5期349-353,共5页
Journal of Clinical Pediatric Surgery
