摘要
目的:观察大鼠脑出血后血肿周围神经元凋亡表达的变化规律及钙拮抗剂-氟桂利嗪的干预作用。方法:实验于2003-03/09在解放军第三军医大学西南医院中心实验室完成。①取66只成年健康Wistar大鼠,随机分为正常组6只、脑出血组30只,氟桂利嗪治疗组30只;脑出血组及氟桂利嗪治疗组又分别于出血后0.5h,6h,24h,72h,120h5个时间点进行观察,每个时间点6只。②造模及给药:运用胶原酶法建立大鼠脑出血模型。氟桂利嗪药粉用生理盐水配成1g/L混悬液,出血前1d及出血后连续5d,氟桂利嗪治疗组大鼠1mg/kg腹腔内注射给药,1次/d。③观察指标:经末端脱氧核糖核苷转移酶介导的DUTP缺口末端标记法和二氨基联苯胺染色,测定脑出血后各时点血肿周围神经元凋亡表达。结果:66只大鼠均进入结果分析。①脑出血后血肿周围凋亡细胞增多,0.5h开始升高(100.88±9.43)个/视野,6h明显增多(171.83±19.07)个/视野,24h达高峰(217.88±22.56)个/视野,72h血肿周围凋亡细胞数逐渐减少(164.50±14.07)个/视野,120h基本接近正常(54.00±12.46)个/视野。出血后各时间点与正常组(27.5±6.95)个/视野]比较差异有显著性意义(P<0.01)。②氟桂利嗪治疗后0.5h,6h,24h,72h,120hTUNEL阳性细胞明显减少,分别为(81.17±11.29)个/视野,(101.67±10.54)个/视野,(129.83±9.28)个/视野,(71.5±16.27)个/视野,(30.67±8.09)个/视野,与脑出血组相应时点比较差异有显著性意义(P<0.01)。结论:氟桂利嗪明显抑制脑出血后各时点血肿周围凋亡表达,对实验性脑出血神经元损伤起保护作用。.
AIM： To observe the changing roles of the expression of neuronal apoptosis around hematoma of rats with intracerebral hemorrhage （ICH） and the intervention effect of calcium antagonist flunarizine .
METHODS： The experiment was conducted in the Central Laboratary, Southwest Hospital of Third Military Medical University from March to September 2003. （1）Sixty-six healthy adult Wistar rats were randomly divided into normal group（n=6）, ICH group （n=30） and flunarizine treatment group （n=30）; the latter two groups were observed in 5 different time-points respectively, such as half an hour, 6 hours, 24 hours, 72 hours and 120 hours after ICH, and six rats were observed in each time-point. （2） Modeling and administration： Models of rats with ICH were made with collagenaseinduced method. Suspension of 1 g/L was prepared by medicine power of flunarizine and normal saline. Rats in the flunarizine group received 1 mg/kg intraperitoneal injection of suspension once a day one day before ICH and for continuous 5 days after ICH.. （3）Observing index： Number of neuronal apoptosis around hematoma after intracerebral hemorrhage of each time-point was measured after being stained with diaminobenzidine （DAB） method and DUTP nicking terminal labelling mediated by terminal deoxyribonucleoside transferase, immunohistochemical
RESULTS： A total of sixty-six rats were involved in the analysis of results. （1）The number of apoptosis cells around hematoma after ICH began to increase. Increased at half an hour （100.88±9.43）, significantly increased at 6^th hour（171.83±19.07）, reached its peak at the 24^th hour （217.88±22.56）, the number gradually decreased from the 72^th hour （164.50±14.07）and got close to normal range at the 120^th hour（54.00±12.46）. There were significant differences between the former two groups in each time-point of ICH and the normal group（27.5±6.95） （P〈0.01）. （2）After treated with flunarizine, the number of apoptotic cells around hematoma in different time-point significantly decreased 81.17±11.29,101.67±10.54,129.83±9.28,71.5±16.27, 30.67±8.09 respectively. There was significant difference in comparing with ICH group in each time-point（ P〈0.01 ）.
CONCLUSION： Flunarizine can inhibite the expression of apoptotic cells around hematoma in each time-point, and has a protective effect on neuronal injury after ICH.
出处
《中国临床康复》
CSCD
北大核心
2005年第45期40-42,i0005,共4页
Chinese Journal of Clinical Rehabilitation
基金
解放军第三军医大学校管基金资助项目(2002)~~
