摘要
目的研究肿瘤转移抑制基因ｎｍ２３，表达产物二磷酸核苷激酶（ＮＤＰＫ）在Ⅲ期卵巢癌中的表达及其意义。方法用微波仪ＡＢＣ免疫组织化学染色法研究了２３例Ⅲ期卵巢癌患者。结果ＮＤＰＫ／ｎｍ２３在人Ⅲ期卵巢癌中有较高的表达，阳性率为５２．１７％（１２／２３），其中无淋巴结转移的阳性率为８１．８２％（９／１１），伴有淋巴结转移的阳性率为２５％（３／１２），差异有显著性（Ｐ＝０．０２）；卵巢癌的细胞分化程度越高，ｎｍ２３的阳性检出率也越高，各级之间差异有显著性（Ｐ＜０．０５）。结论ｎｍ２３基因可能在卵巢癌转移和细胞分化过程中起调节作用，其表达程度与卵巢癌淋巴结转移呈负相关，与其分化程度呈正相关。
To detect the expression of nucleotide diphosphate kinase (NDPK), the product of metastasis suppressor gene nm23in the human ovarian carcinoma of three stages.Methods Microwave instrument ABC immunohistochemical method was used.Results 23 patients were tested, 12(52. 17 %) showed positive staining. Higher incidence of positive staining was found in ovarian carcinoma without lymph node metastasis (91/11, 81. 82%) than in that with lymph involvement (3/12 , 25 %). (P = 0. 02) . The nm23 gene was expressed at higher levels in well differentiated tumors (p<0. 05).Conclusions NDPK/nm23 may play different roles in the metastasis and cell differentiation of human ovarian carcinoma. Its expression levels are not only inversely correlated with lymph node metastasis,but also positively correlated with histological grading of ovarian carcinoma.
出处
《现代妇产科进展》
CSCD
1996年第2期107-109,共3页
Progress in Obstetrics and Gynecology
关键词
卵巢肿瘤
肿瘤转移抑制
基因
基因表达
淋巴转移
Ovarian neoplasms
Gene expression
Lymph node metastasis
Immunohistochemistry
