摘要
目的:研究p16,p27基因在卵巢上皮性恶性肿瘤中的表达及其临床意义.方法:用免疫组织化学方法检测18例卵巢正常组织、20例卵巢良性肿瘤及38例卵巢癌组织中p16,p27基因的表达情况,并与临床特征相比较.结果:P16蛋白在卵巢上皮性恶、良性肿瘤和正常组织中的阳性表达率分别为39%,70%和83%;P27蛋白在卵巢上皮性恶、良性肿瘤和正常组织中阳性表达率分别为32%,65%和78%,P16和P27蛋白在卵巢上皮性恶性肿瘤中的表达明显低于卵巢正常组织和卵巢良性肿瘤.在细胞分化程度Ⅰ级的肿瘤中,P16和P27蛋白表达阳性率明显高于分化程度Ⅱ级和Ⅲ级的肿瘤(P<0.01);在临床分期早期的肿瘤中,P16和P27蛋白的表达率明显高于临床分期晚期的肿瘤(P<0.01).不伴淋巴转移的P16和P27蛋白的表达率明显高于有淋巴转移的蛋白的表达率(P<0.01).结论:p16,p27基因的表达与卵巢上皮性恶性肿瘤的细胞分化程度、临床分期及有无淋巴转移密切相关.临床检测卵巢上皮性肿瘤P16和P27蛋白的表达,可以作为判断卵巢上皮性恶性肿瘤生物学行为及患者预后的参考指标.
AIM： To study the expressions of P16 and P27 and its clinical significance in epithelial ovarian carcinoma. METHODS： Archival ovarian epithelial carcinoma （n = 38 ）, normal ovarian issues（ n = 18） and benign ovarian tumor （ n = 20 ） were detected for P16 and P27 expressions by immunohistochemistry streptavidin-peroxidase staining （ SP method）. RESULTS： The positive rates of P16 expression in ovarian epithelial carcinoma, benign ovarian tumor and normal tissues were 39%, 70% and 83% respectively and the positive rates of P27 expression in ovarian epithelial carcinoma, benign ovarian tumor and normal tissues were 32% ,65% and 78% respectively. The P16 and P27 expressions in ovarian epithelial carcinoma were obviously lower than those in benign ovarian tumor and in normal tissues. In histological grades of ovarian epithelial carcinoma, the rates of high P16 and P27 protein expressions in well-differentiated samples were significantly higher than those in moderately-differentiated samples and poorly-differentiated samples （P 〈 0.01 ）. The rates of high P16 and P27 protein expressions in early clinical stage of ovarian epithelial carcinomas were significantly higher than those in late clinical stage （ P 〈0.01 ）. The rates of high P16 and P27 protein expressions in ovarian epithelial carcinomas with lymph node metastasis were significantly lower than those without lymph node metastasis （ P 〈 0.01 ）. CONCLUSION ： The expressions of P16 and P27 protein are correlated to the histological degree of cancer cells, clinical phase of the patients and lymph node metastasis. The detection of P16 and P27 protein expressions may be a valuable marker for the tumor malignant behavior and the prognosis of the patients with ovarian epithelial carcinoma.
出处
《第四军医大学学报》
CAS
北大核心
2006年第1期30-33,共4页
Journal of the Fourth Military Medical University
