摘要
目的:验证骨髓基质干细胞(MSC)移植到缺血心肌中后是否在心肌的微环境中可以向心肌细胞分化,提高心功能.方法:采用自体MSC体外培养扩增移植.在通过结扎冠状动脉造成急性心肌缺血后,被5溴-2脱氧尿苷(BrdU)标记后的MSC移植到自体的缺血心肌中.结果:移植4wk后,MSC向肌细胞分化,表达出α-横纹肌肌动蛋白(sarcomericac-tin)和存在于闰盘中的connexin43,移植后心肌表达VEGF增加[(684±86)fg/Lvs(515±51)fg/L,P<0.05],缺血心肌局部血管密度增加[(22.9±6.6)/HPvs(19.0±5.9)/HP,P<0.05],左室收缩功能明显强于对照组[LVSP:(15.08±0.84)kPavs(13.26±0.68)kPa;LVEDP:(1.53±0.28)kPavs(19.03±0.41)kPa;+dp/dtmax:(+615.77±48.69)kPa/svs(+435.75±58.25)kPa/s;-dp/dtmax:(-401.17±46.23)kPa/svs(-338.25±50.72)kPa/s,P<0.05].结论:MSC移植可治疗心肌缺血,并且没有免疫排斥反应.
AIM： To test the hypothesis that marrow stromal cells （ MSCs）, when implanted into self-myocardium, can undergo milieu-dependent differentiation, express cardiomyogenic phenotypes and enhance angiogenesis and cardiac function of ischemic hearts in vivo. METHODS： Acute myocardial infarction was induced by occlusion of left anterior descending artery, and autologous MSCs labeled with BrdU （Bromodeoxyurldine） in vitro were administrated intramyocardially into the infarct area of the same donor rabbits, RESULTS： After 4 weeks, transplanted MSCs demonstrated myogenic differentiation with the expression of α- sarcomeric actin and connexin 43. MSCs displayed increased levels of VEGF protein [ （684 ±86） fg/L vs （515 ±51 ） fg/L, P〈 0.05 ] and the number of vessels [ （ 22.9 ± 6.6 ）/HP vs （ 19.0 ± 5.9）/HP, P〈 0. 05 ] in myocardial ischemia area, compared with those in controls. MSCs implantation resulted in markedly improved left ventricular contractility [ LVSP： （ 15.08 ± 0. 84 ） kPavs （13.26 ±0.68） kPa; LVEDP： （1.53 ±0.28） kPa vs （19.03±0.41） kPa; ＋dp/dt max： （＋615.77 ±48.69） kPa/s vs （ ＋435. 75 ±58. 25） kPa/s; - dp/dt max： （ -401. 17 ± 46.23） kPa/s vs （ -338.25 ±50. 72） kPa/s, P〈0. 05]. CONCLUSION： Autologous MSCs transplantation can effectively treat myocardial ischemia without immune rejection.
出处
《第四军医大学学报》
北大核心
2006年第1期19-22,共4页
Journal of the Fourth Military Medical University
关键词
骨髓基质干细胞
缺血心肌
移植
marrow stromal cell
ischemic myocardium
transplantation
