期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

骨形成蛋白2基因修饰的老年大鼠骨髓间充质干细胞成骨分化能力的研究 被引量：8

STUDY ON THE DIFFERENTIATION OF MARROW MESENCHYMAL STEM CELLS OF THE RATS MEDIATED BY BONE MORPHOGENETIC PROTEIN 2
暂未订购
导出
摘要 目的观察年龄因素对骨髓间充质干细胞(marrowmesenchymalstemcells,MSCs)成骨分化能力的影响;了解基因治疗对老年大鼠MSCs成骨分化能力的影响。方法1月龄(幼年组)、9月龄(成年组)及24月龄(老年组)雄性Wistar大鼠各6只,取MSCs经体外分离、培养及携带骨形成蛋白2(bonemorphogeneticprotein2,BMP-2)基因的腺病毒载体(Ad-BMP-2)转染后,定量检测BMP-2、碱性磷酸酶(alkalinephosphate,ALP)表达,以及成骨细胞标志性蛋白:型胶原、骨涎蛋白(bonesialoprotein,BSP)和骨桥素(osteopontin,OPN)的表达。将转染的各组MSCs分别与磷酸三钙(tricalciumphosphate,TCP)复合后植入裸鼠体内,3周后取材,比较各组诱导异位成骨能力。结果ELISA检测表明BMP-2基因修饰的MSCs可以有效表达BMP-2,且表达量在各年龄组间差异无统计学意义(P>0.05);各组ALP于诱导后第9天达高峰,但组间差异均无统计学意义(P>0.05);诱导后第7天,RT-PCR半定量检测示各组均有成骨细胞特征性蛋白,即:型胶原、OPN及BSP的明显表达,表达量在各组间差异无统计学意义(P>0.05);BMP-2基因转染的MSCs与TCP复合后可诱导裸鼠体内异位成骨,各组成骨量差异无统计学意义(P>0.05)。结论BMP-2基因修饰的老年大鼠MSCs可以恢复成骨分化能力,基因治疗可能为老年性骨骼疾病提供一种新的治疗途径。 Objective To explore the effect of age and gene therapy on the differentiation of marrow mesenchymal stem cells （MSCs） of the rats, Methods MSCs from the young （1-month old）, adult （9-month old）, and the aged （24-month-old） rats were expanded in culture and infected with adenovirus mediated human bone morphogenetic protein 2 gene （Ad-BMP-2）, The expression of BMP-2 and osteoblastic markers such as alkaline phosphatase （ALP）, co[[agen Ⅰ （Col-Ⅰ）, bone sialoprotein （BSP） and osteopontin （OPN） were assayed during the process of differentiation, Their abilities to induce ectopic bone formation in nude mice were also tested, Results There was no significant difference in the expression of BMP 2 among the 3 groups, ALP activity assay and semi quantitative reverse transcription polymerase chain reaction（RT-PCR） demonstrated that there were no significant differences in the expression of osteoblastic markers ALP, Col-Ⅰ , OPN and BSP among the 3 groups, Histomorphometric analysis indicated that there were no significant differences in the volume of the newly formed ectopic bones in nude mice among the 3 groups, Conclusion MSCs obtained from the aged rats can restore their osteogenic activity following human BMP-2 gene transduction,therefore provides an alternative to treating the aged bone disease,
作者 岳冰 陈燕飞 汤亭亭 陆斌 郁朝锋 楼觉人 戴戎
机构地区 上海交通大学医学院附属第九人民医院骨科 太原师范学院生物系 美国华盛顿大学医学院骨科实验室
出处 《中国修复重建外科杂志》 CAS CSCD 北大核心 2006年第1期53-57,共5页 Chinese Journal of Reparative and Reconstructive Surgery
基金 国际合作资助项目(014307021) 上海市科委资助项目(024119016)~~
关键词 老年 骨髓间充质干细胞 骨形成蛋白2 基因治疗 大鼠 The aged Marrow mesenchymal stem cells Bone morphogenetic protein 2 Genetherapy Rat
分类号 R68 [医药卫生—骨科学]
  • 相关文献

参考文献13

  • 1[1]Lu JX,Gallur A,Flautre B,et al.Comparative study of tissue reactions to calcium phosphate ceramics among cancellous,cortical,and medullar bone sites in rabbits.J Biomed Mater Res,1998,42(3):357-367.
  • 2刘继中,纪宗玲,胡蕴玉.腺病毒介导的基因治疗在骨创伤及骨病中的应用[J].中国修复重建外科杂志,2003,17(3):215-218. 被引量：6
  • 3[3]Shur I,Lokiec F,Bleiberg I,et al.Differential gene expression of cultured human osteoblasts.J Cell Biochem,2001,83(4):547-553.
  • 4[4]Quarto R,Thomas D,Liang CT.Bone progenitor cell deficits and the age-associated decline in bone repair capacity.Calcif Tissue Int,1995,56(2):123-129.
  • 5[5]Leskela HV,Risteli J,Niskanen S,et al.Osteoblast recruitment from stem cells does not decrease by age at late adulthood.Biochem Biophys Res Commun,2003,311(4):1008-1013.
  • 6[6]Stenderup K,Justesen J,Clausen C,et al.Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells.Bone,2003,33(6):919-926.
  • 7[7]Bergman RJ,Gazit D,Kahn AJ,et al.Age-related changes in osteogenic stem cells in mice.J Bone Miner Res,1996,11(5):568-577.
  • 8[8]D'Ippolito G,Schiller PC,Ricordi C,et al.Age-related osteogenic potential of mesenchymal stromal stem cells from human vertebral bone marrow.J Bone Miner Res,1999,14(7):1115-1122.
  • 9[9]Nishida S,Endo N,Yamagiwa H,et al.Number of osteoprogenitor cells in human bone marrow markedly decreases after skeletal maturation.J Bone Miner Metab,1999,17(3):171-177.
  • 10[10]Beresford JN,Bennett JH,Devlin C,et al.Evidence for an inverse relationship between the differentiation of adipocytic and osteogenic cells in rat marrow stromal cell cultures.J Cell Sci,1992,102 (Pt 2):341-351.

二级参考文献25

  • 1Simonet WS,Lacey DL,Dunstan CR,et al.Osteoprotegerin:A novel secreted protein involved in the regulation of bone density.Cell,1997; 89(2):309.
  • 2Bekker PJ.Holloway D,Nakanishi A.et al.The effect of a single dose of osteoprotegerin in postmenopausal women.J Bone Miner Res,2001；16(2):348.
  • 3Rodan GA,Martin TJ.Therapeutic approaches to bone diseases.Science,2000；289(5484):1508.
  • 4Jolly D.Viral vector systems for gene therapy.Cancer Gene Ther,1994; 1(1):51.
  • 5Lieberman JR,Daluiski A,Stevenson S,et a1.The effect of regional gene therapy with bone morphogenetic protein-2-producing bone—marrow cells on the repair of segmental femoral defects in rats.J Bone Joint Surg(Am),1999; 81(7):905.
  • 6Sommer B,Kuznetsov SA,Robey PG,et al.Efficient gene transfer into normal human skeletal cells using recombinant adenovirus and Conjugated adenovkus—DNA complexes.Calcif Tissue Int,1999；64(1):45.
  • 7Nishida K,Kang JD,Suh JK,et al.Adenovirus—mediated gene transfer to nucleus pulposus cells.Implications for the treatment of intervertebral disc degeneration.Spine,1998；1523(22):2437.
  • 8Tanaka S.Takahashi T,Takayanagi H,et al.Modulation of osteoclast function by adenovirus vector—induced epidermal growth factor receptor.J Bone Miner Res,1998; 13(11):1714.
  • 9Okubo Y,Bessho K,Fujimura K,et al.In vitro and in vivo studies of a bone morphogenetic protein-2 expressing adenoviral vector.J Bone Joint Surg(Am),2001；83(Suppl):99.
  • 10Conget PA,Minguell JJ.Adenoviral-mediated gene transfer into ex vivo expanded human bone marrow mesenchymal progenitor cells.Exp Hematol,2000；28(4):382.

共引文献5

同被引文献128

引证文献8

二级引证文献50

中国修复重建外科杂志
2006年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部