摘要
目的:研制抗金葡菌多肽单克隆抗体(mA b)。方法:抗金葡菌多肽(PH-SA)抗原采用脾内注射、醋酸纤维膜皮下包埋、完全佐剂3种方法免疫Balb/C小鼠,常规方法细胞融合,用EL ISA间接法筛选阳性细胞并测定相对亲和力,双抗体竞争试验和双抗体竞争抑制试验识别PH-SA表位。结果:获4株可分泌PH-SA mA b的杂交瘤细胞株。免疫球蛋白亚类测定均为小鼠IgG1亚类,相对亲和力依次为G11>B9>H3,4株mA b可能抗相同抗原表位。结论:获4株PH-SA mA b的杂交瘤细胞株为PH-SA的鉴定提供了可能性。
Objective： To obtain monoclonal antibodies （mAbs） against Pheromonicin-and-Staphylococcus aureus （PH-SA）. Methods： BALB/c mice were immunized by immunogen PH-SA in three different ways-intrasplenic inoculation, subcutaneous cellulose acetate membrane embedding, and complete Freud＇s adjuvants. Splenocytes of immunized mice and Sp2/0 cells were fused conventionally, and indirect ELISA was applied to screen for positive mAb-secreting hybridoma cells and detect mAb relative affinity. The epitopes on PH-SA recognized were analyzed by double antibody competition test and double antibody competition inhibition test. Results： Four hybridoma cell lines secreting mAbs against PH-SA, all of IgG1 subclass, were obtained, and the relative affinity lies in G11〉B9〉H3. the antibodies might recognize the same epitope on PH-SA. Conclusion： The four obtained hybridoma cell lines secreting mAbs against PH- SA provide the possibility of PH-SA identification.
出处
《陕西医学杂志》
CAS
北大核心
2006年第1期6-7,32,共3页
Shaanxi Medical Journal
基金
国家"十五"重大科技专项"创新药物和中药现代化"基金(No 2002AA2Z3350)
关键词
抗生素类
肽
抗体
单克隆
相对亲和力
表位
Antibiotics,peptide Antibodies monoclonal Relative affinity Epitope
