摘要
目的:通过对角膜上皮损伤后病理改变的动物实验观察,了解海水浸泡对伤后上皮组织愈合的影响。方法:对10只灰兔表麻下刮除双侧全角膜上皮,右眼为实验眼,左眼为对照眼。实验眼海水浸泡半小时,对照眼生理盐水浸泡半小时。分别于伤前和伤后1、3、5、7、10d进行角膜厚度测定、角膜上皮荧光染色、裂隙灯下检查。按期依序处死2只实验兔,取全眼球甲醛固定后,石蜡包埋切片,常规染色,光镜下观察。角膜厚度值分别进行伤前与伤后自身对照t检验,实验眼和对照眼t检验。结果:角膜去上皮损伤后,裂隙灯下见伤后角膜明显水肿混浊并逐日加重,且实验眼较对照眼严重,实验眼伤后10d可见角膜缘有大量新生血管长入。角膜上皮荧光染色检查见,伤后角膜上皮均呈片状着色,但实验眼明显大于对照眼。光学显微镜下见实验眼角膜组织炎性反应明显加重,上皮细胞愈合明显延迟,基质血管化加重。伤后角膜厚度均较伤前明显增加,实验眼角膜厚度增加更为明显,伤后角膜与伤前角膜自身对照t检验和实验眼与对照眼伤后角膜厚度经分组t检验,均有显著差异。结论:海水浸泡对伤后上皮组织的愈合有不良影响。
Purpose：Use slit lamp microscope and optical microscope to observe the changes in corneal tissue after corneal epidermis injury and the effect of seawater soaking on the healing of eorea after explosive injury.Method：Corneal epidermis were scraped on 20 eyes of 10 adult grey rabbits.For each rabbit, the right eye was in experiment and the left one was for comparison.After the injury,the experimental eyes were soaked in seawater for 30 minutes and the comparable eyes were soaked in 0.9% sodium chloride injection for the same time.All eyes were underwent slit lamp examinations, fluorescein dyeing, and ultrasonic cornea thickness metering on the corneas before the injury and 1,3,5,7,10 days after the injury respectively.At the same time,2 rabbits were killed and the eyes were examined with pathology.The cornea thickness numbers were compared using selfcomparison t test and grouping t test.Result：After scraping the whole corneal epidermis, slit lamp microscope examination found that the corneas were edema and turbidity, the experimental eyes were stricter than the compaxable eyes.The corneas of the experimental eye were vascularized 10 days after the injury.Fluorescein dyeing showed that more apparent pigmentation was found on the experimental eyes than that on the comparable ones.Comparative examination under the optical microscope showed that the corneal tissue of the experiment eyes changed distinetly,its inflammation is patency and healing was delayed and the vasenlarization degree became high after the injury.Cornea thickness of the experimental eyes became apparently thicker than that of the comparable eyes.The diffcrence was statistically significant.Conclusion：Seawater soaking will have bad effects on the healing of the corneal epidermis injury.Eye Science 2005;21：149-152
出处
《眼科学报》
2005年第4期149-152,共4页
Eye Science
关键词
角膜上皮
外伤
海水
病理
corneal epidermis
injury
seawate
pathology
