摘要
体外培养少突胶质细胞已证明其细胞学特征与在体的发育相近[1~3],而且改变了以前对少突胶质细胞突起较少的错误认识.少突胶质细胞的形态、功能、发育以及与神经元的相互联系逐渐被神经科学工作者所重视,而其前体细胞的增殖和纯化是进一步研究的基础.少突胶质前体细胞的连续发育过程报道也较少.本室在既往研究的基础上对少突胶质前体细胞的体外培养方法进行改良,然后对少突胶质细胞系部分发育阶段进行了形态学观察,以期为深入了解少突胶质细胞系的细胞生物学特性及探索发育神经生物学的规律提供帮助.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2005年第6期718-721,共4页
Chinese Journal of Anatomy
参考文献14
-
1Knapp P E,Bartlett W P,Skoff R P.Cultured oligodendrocytes mimic in vivo phenotypic characterisitics:Cell shape,expression of myelin-specific antigens and membrane prodution.Dev Biol,1987,120:356-365.
-
2Agresti C,Meomartini ME,Amadio S,et al.ATP regulates oligodendrocyte progenitor migration,proliferation,and differentiation:involvement of metabotropic P2 receptors.Brain Res Brain,2005,48(2):157-165.
-
3Kakinuma Y,Saito F,Ohsawa S,et al.A sulfatase regulating the migratory potency of oligodendrocyte progenitor cells through tyrosine phosphorylation of beta-catenin.J Neurosci Res,2004,77(5) :653-661.
-
4Zhang SC,Ge B,Duncan ID.Adult brain retains the potential to generate oligodendroglial progenitors with extensive myelination capacity.Proc Natl Acad Sci USA,1999,96(7) :4089-4094.
-
5Reynolds R,Wilkin GP.Development of macroglial cells in rat cerebellum Ⅱ:an in situ immunohistochemical study of oligodendroglial lineage from precursor to mature myelinating cell.Development,1988,102(2) :409-425.
-
6Levison SM,Goldman JE.Multipotential and lineage restricted precursors coexist in the mammalian perinatal subventricular zone.J Neurosci Res,1997,48(2) :83-94.
-
7Armstrong RC.Isolation and characterization of immature oligodendrocyte lineage cells.Methods,1998,16(3) :282-292.
-
8Barres BA,Raff MC,Gaese F,et al.A crucial role for neurotrophin-3 in oligodendrocyte development.Nature,1994,367(6461) :371-375.
-
9Kondo T,Raff M.Oligodendrocyte precursor cells reprogrammed to become multipotential CNS stem cells.Science,2000,289(5485):1754-1757.
-
10Rousselet A,Autillo-Touati A,Araud D,et al.In vitro regulation of neuronal morphaogenesis and polarity by astrocyte-derived factors.Dev Biol,1990,137(1) :33-45.
共引文献39
-
1马丽香,李瑞锡,王劼,汪洋,彭裕文.低氧诱导体外培养大鼠星形胶质细胞VEGF的表达[J].解剖学研究,2004,26(3):177-179. 被引量:1
-
2马丽香,王劼.多重免疫荧光标记法在神经胶质细胞研究中的应用[J].解剖学研究,2004,26(3):237-238. 被引量:1
-
3安珂,田玉科,杨辉,高峰,王鹏.猿肾病毒40大T抗原基因永生化大鼠星形胶质细胞株的构建[J].中华麻醉学杂志,2004,24(11):838-841. 被引量:11
-
4宁章勇,赵德明,杨建民,崔亚利,周向梅,秦秀慧.大鼠大脑皮质星形胶质细胞体外分离培养和纯化[J].中国兽医杂志,2005,41(9):9-11. 被引量:1
-
5付元山,王滨,汪洋,刘才栋,沈馨亚.混合培养对O2A祖细胞定向分化的影响[J].中国临床神经科学,2005,13(4):402-405.
-
6付元山,王滨,汪洋,刘才栋,沈馨亚.大鼠少突胶质前体细胞在化学条件培养基中定向分化的形态学观察[J].中国临床康复,2006,10(1):15-17. 被引量:1
-
7张斌,魏欣冰,刘慧青,王立祥,孙茹,张岫美.羟乙基葛根素对脑星形胶质细胞氧化性损伤的保护作用[J].药学学报,2006,41(2):171-174. 被引量:16
-
8钱琼英,王劼,李瑞锡,林芮禾,马丽香,彭裕文.低氧刺激诱导体外培养的大鼠脑皮质星形胶质细胞内neuregulin-1表达上调[J].神经解剖学杂志,2006,22(2):141-146. 被引量:2
-
9杨勤,沈馨亚,刘才栋,严恒林,汪洋.层粘蛋白对雪旺细胞在三维网架上迁移的影响[J].解剖学杂志,1996,19(5):400-403. 被引量:13
-
10杨勤,沈馨亚,刘才栋,严恒林,汪洋.雪旺细胞在聚酯纤维上的迁移及对神经突起的引导作用[J].解剖学杂志,1996,19(5):404-407. 被引量:10
同被引文献13
-
1Reynolds R,Wilkin GP.Development of macroglial cells in rat cerebellum Ⅱ:an in situ immunohistochemical study of oligodendroglial lineage from precursor to mature myelinating cell.Development,1988,102(2) :409-425.
-
2Levison SM,Goldman JE.Multipotential and lineage restricted precursors coexist in the mammalian perinatal subventricular zone.J Neurosci Res,1997,48(2):83-94.
-
3Agresti C,Meomartini ME,Amadio S,et al.ATP regulates oligodendrocyte progenitor migration,proliferation,and differentiation:involvement of metabotropic P2 receptors.Brain Res Brain,2005,48(2) :157-165.
-
4Kakinuma Y,Saito F,Ohsawa S,et al.A sulfatase regulating the migratory potency of oligodendrocyte progenitor cells through tyrosine phosphorylation of beta-catenin.J Neurosci Res,2004,77(5):653-661.
-
5Varanl J,Orr W,Ward PA.A comparison of the migration patterns of normal and malignant cells in two assay systems.Am J Pathol,1978,90(1):159-171.
-
6Asakura K,Hunter SF,Rodriguez M.Effects of transforming growth factor-beta and platelet-derived growth factor on oligodendrocyte precursors:insights gained from a neuronal cell line.J Neurochem,1997,68(6) :2281-2290.
-
7Raff MC,Lillien LE,Richardson WD,et al.Platelet-derived growth factor from astrocytes drives the clock that times oligodendrocyte development in culture.Nature,1988,333 (6173) :562-565.
-
8Mckinnon RD,Matsui T,Dubois-Dalcq M,et al.FGF modulates the PDGF-driven pathway of oligodendrocyte development.Neuron,1990,5(5) :603-614.
-
9Armstrong RC,Harvath L,Duleq ME.Type-1 astrocytes and oligodendrobyte-type-2 astrocyte glial progenitors migrate toward distinct molecules.J Neurosci Res,1990,27(3):400-407.
-
10Decker L,Avellana-Adalid V,Nait-Oumesmar B,et al.Oligodendrocyte precursor migration and differentiation:Combined effects of PSA residues,Growth factors,and substrates.Mol Cell Neurosci,2000,116(4):422-439.
-
1王颖.认知行为干预对糖尿病(DM)患者治疗依从性的影响[J].当代医学,2015,21(14):61-62. 被引量:4
-
2李珊.对癫痫的十种错误认识[J].求医问药,2008(9):16-18.
-
3曾凡龙,王燕,郭鄂平.旋毛虫细胞学特征初探[J].医学动物防制,2004,20(8):486-487.
-
4张燕,梁元晶.胚胎和成体神经干细胞分化机制的研究[J].生物学通报,2005,40(2):8-10.
-
5钟碧贤.揭开心理咨询的面纱[J].心血管病防治知识,2010(10):66-66.
-
6程柯.对脑卒中的几种错误认识[J].求医问药,2011(5):9-9.
-
7邓锦波,蔡琰.干细胞与神经元、神经胶质细胞的分化[J].解剖科学进展,1995,1(4):301-305.
-
8梁建华,孙义华,宫殿荣,张广珠,李兴文.脊髓蛛网膜炎的脑脊液细胞学[J].中国航天工业医药,2001,3(4):27-28.
-
9李媛媛.花季少女的“私密地图”[J].家庭医学(上半月),2005,20(2):21-22.
-
10姚缨,史则峡.妊娠剧吐并发Wernicke脑病3例临床分析[J].实用妇产科杂志,2005,21(2):118-119. 被引量:11