摘要
目的探讨线粒体DNA与人宫颈细胞癌变的关系。方法采用PCR方法制备地高辛标记的3条人mtDNA探针,对一株人宫颈癌Hela细胞系1、例原代培养人皮肤成纤维细胞和24例子宫颈癌患者的活检癌组织及癌旁宫颈组织的染色体或间期细胞核进行了荧光原位杂交分析。结果3条mtDNA探针在原代培养人皮肤成纤维细胞染色体或间期核中均未出现阳性杂交信号;而在人宫颈癌Hela细胞和24例肿瘤组织标本的部分染色体或间期细胞核中均出现了阳性杂交信号。而在癌旁组织的部分染色体或间期细胞核中虽也出现了阳性杂交信号。但与肿瘤组织标本相比有显著性差异。结论表明癌细胞核基因组中存在mtDNA的同源序列,这些同源片段的出现可能与细胞癌变有关。
Objective： To explore the relationship between mitochondrion DNA （mtDNA） and carcinogenesis of human Cervical cells. Methods：Three kinds of non- radioactive DIG- labelled, mtDNA probes were prepared by polymerase chain reaction （PCR） . The chromosomes in metaphase or interphase nuclei of one fibrobasts and Hela cell line and 24 cervical carcinomas together with the adjacent normal tissues were analyzed by fluorescence in situ hybridization （FISH）. Results： Hybridization signals were4 partly observed on the chromosomes in metaphase or interphase nuclei of Hela cell line and 24 cervical carcinomas together with the adjacent normal tissues but not in fibrobasts. Conclusion： The mtDNA homology sequence exists in the nuclear genome of carcinomas cells and it might exert important effects on the cqarcinogenic process.
出处
《中国优生与遗传杂志》
2005年第12期17-18,共2页
Chinese Journal of Birth Health & Heredity
关键词
线粒体DNA探针
宫颈癌
核基因组
荧光原位杂交
Mitochondrion DNA probe
Cervical carcinomas
Nnuclear genome
Fluorescence in situ hybridization
