摘要
目的建立一种快速准确的发砷含量测定方法.方法人发经HNO3-HClO4-H2SO4混合酸消化、电热板加热处理后,在5%盐酸酸度下应用氢化物发生-原子荧光光谱仪(HG-AFS)测定人发中砷含量.结果砷浓度在0～200 μg/L范围内线性关系良好,r≥0.9995.测定高、中、低3种浓度标准液各15次,相对标准偏差分别为1.0%、1.3%、1.9%,加标回收率分别为94.41%、94.91%、101.17%.对国家标准物质中心的人发样(GBW09101)进行平行测定,结果均在规定范围内;检出限0.0728 μg/L;发中Zn、Cu、Fe、Mn离子对发砷含量的测定并无显著影响.处理后的样品在4℃冰箱或20℃室温下可稳定保存一周.应用该法对187名离岗4年的某冶炼厂职工进行发砷含量测定,检测结果0.002～5.77 μg/g,平均值为0.5 μg/g,其中3人发砷含量高于5 μg/g正常参考值.结论该法测定发砷含量,具有精密度好,检出限低,线性范围宽,受干扰小等优点,是实用性很强的高效低耗分析技术,适用于大批量发样的快速测定.
Objective To establish a fast and accurate method for determining the arsenic content in human hair. Method After digesting with the mixed acids of HNO3-HClO4-H2SO4 and heating by electric hot plate, the arsenic content in human hair was determined under 5% hydrochloric acid acidity by hydride generation-atomic fluorescence spectrometry. Result The results showed that there was a good linear relation when arsenic contents were between 0μg/L to 200μg/L （r≥0.9995） ; the 15 time repeated measurements for high, middle and low contents of arsenic in human hair, the RSD （relative standard deviation） were 1.0%, 1.3% and 1.9%, and their recovery rates were 94.14%, 94.91% and 101.17% respectively. The detection of standard hmnan hair sample （GBW09101） provided by National Standard Substances Center showed the results were all within the acceptable range; the detecting limit was 0.0728μg/L; the existence of zinc, copper, iron and manganese in human hair did not produce any significant influence on determination of arsenic content; the disposed samples could be well stored for one week under 20℃ room temperature or in 4℃ refrigerator. In this study, 187 workers of a metal-smelting factory were measured by this method, four years after leaving the factory, the mean value of the hair arsenic was 0.5μg/g （0.002 μg/g to 5.77 μg/g）, 3 persons among those workers were over the reference value 5 μg/g. Conclusion This method shows many outstanding advantages such as good precision, lower detecting limit, wide linearity range and better stability, it is really a good and practical analytical technique with high efficiency and low wastage, that would be particularly suitable for the quick determination of the large quantities of hair samples.
出处
《中国工业医学杂志》
CAS
北大核心
2005年第6期331-333,共3页
Chinese Journal of Industrial Medicine
关键词
氢化物发生-原子荧光光谱法
发砷
Hydride generation-atomic fluorescence spectrometry
Human hair
Arsenic contents in human hair
