摘要
目的研究三七总皂甙(PNS)对大鼠移植肝缺血再灌注损伤中保护作用。方法改良Kam ada法建立同种大鼠原位肝脏移植模型,将动物模型随机分为3组:生理盐水对照组(N组)、三七总皂甙预处理组(P组)和假手术组(S组)。3组分别于供肝再灌注后2、6、24h时相点,用免疫组化检测NF-κB及ICAM-1的表达;用髓过氧化酶法(MPO)定量测定肝组织中中性粒细胞浸润,并抽血检测血清丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)水平。结果N组供肝再灌注后2h NF-κB的活性显著升高,之后逐渐下降;肝组织ICAM-1的表达在再灌注后2 h开始增高,6h达到高峰,与中性粒细胞的浸润增加和肝损伤有相关性。但P组明显低于N组(P<0.05)。结论大鼠移植肝缺血再灌注时刺激NF-κB的活化,启动ICAM-1的表达参与肝脏缺血-再灌注损伤的发生过程,三七总皂甙能显著降低再灌注时供肝组织中炎症介质的转录表达,并有效改善供肝的再灌注损伤。
Objective To investigate the protection of panax notoginseng saponins（PNS） against ischemia reperfusion injury by liver graft in rats. Method The experimental rat model of orthotopie liver transplantation was set up according to Kamada＇s technique and the rats were randomly divided into three groups： normal saline control group（ group N ）, PNS preconditioning group（ group P） and sham -oper- ated groups（ group S）. The expression levels of NF - κB and intercellular adhesion molecule - 1 （ ICAM - 1 ） were detected by immunohisto- chemistry and the filtration of polymorphonuclear neutrophils（PMNs） was measured with myeloperoxidase（MPO） enzyme method in 2h, 6h and 24h after ischemia reperfusion respectively. The activities of alanine aminotransferase（ALT） and aspartate aminotransferase（AST） in the serum were also detected. Result The activation of NF -κB in group N increased significantly at 2h after reperfusion and then decreased. The expression level of ICAM - 1 increased significantly at 2h after reperfusion, and reached its peak level at 6h in group N. The expression level of ICAM - 1 was significantly lower in group P than that in group N（ P 〈0. 05）. The ICAM - 1 level was positively related with the fil- tration of PMNs and the injury of liver graft. Conclusion The activation of NF - κB and the expression of ICAM - 1 are induced by ischemia reperfusion in rat liver transplantation. The transcription and expression of inflammation media decrease significantly with the treatment of PNS and the ischemia reperfusion injury by liver graft in rats is improved.
出处
《中国医师杂志》
CAS
2005年第12期1628-1630,共3页
Journal of Chinese Physician
