摘要
目的观察肾上腺髓质素ａｄｒｅｎｏｍｏｄｕｌｉｎ，ＡＭ）对血管平滑肌细胞（ＶＳＭＣ）内丝裂素活化蛋白激酶（ｍｉｔｏｇｅｎ－ａｃｔｉｖａｔｅｄｐｒｏ－ｔｅｉｎｋｉｎａｓｅ，ＭＡＰＫ）活性和ＶＳＭＣ增殖的作用。方法测定培养的ＶＳＭＣ内ＭＡＰＫ活性和３Ｈ－ＴｄＲ参入。结果１０－８ＥＴ－１可明显增加培养的ＶＳＭＣｓ内３Ｈ－ＴｄＲ参入（增加６７％，Ｐ＜０．０１）和ＡＰＫ激活（活性增高１１倍，Ｐ＜０．０１），而１０－１０～１０－７ｍｏｌ／Ｌ肾上腺髓质素（１３－５２）［ＡＭ（１３－５２）］与ＥＴ－１（１０－８ｍｏｌ／Ｌ）同时孵育，则可显著抑制ＥＴ－１的上述作用，与１０－８ｍｏｌ／ＬＥＴ－１单独应用时相比，３Ｈ－ＴｄＲ参入和ＭＡＰＫ活性分别减低２２％～５２％（Ｐ值均＜０．０１）和８．１～２９．４％（Ｐ值分别＜０．０５或０．０１）。而单用ＡＭ（１３－５２）对培养的ＶＳＭＣ３Ｈ－ＴｄＲ参入和ＭＡＰＫ活性无明显影响（Ｐ值＞０．０５）。结论ＡＭ（１３－５２）具有抑制ＥＴ所致的ＭＡＰＫ激活和ＶＳＭＣ增殖的作用。ＡＭ与ＥＴ相互作用，参与内皮源的细胞旁分泌调节体系的构成。
AimToobservetheefectofa-drenomodulin(AM)onendothelin(ET)-inducedmi-to
gen-activatedproteinkinase(MAPK)activationwithinthevascularsmoothmusc
lecels(VSMCs)andVSMCproliferation.MethodsTheintracelularMAPKactivitya
nd3H-TdRincorporationwasmea-suredintheculturedVSMCstreatedwithET-1,AM
(13-52),ET-1+AM(13-52),respectively.ResultsThisstudydemonstratesthatE
T-1(10-8mol/L)significantlyincreasestheculturedVSMCs3H-TdRincorporati
on(P<0.01)andMAPKactivity(P<0.01).AM(13-52)(10-10～
10-7mol/L)markedlyin-hibitstheET-1-stimulated3H-TdRincorporationandMA
PKactivationinaconcentration-dependentman-ner.Thedecreasesare22.4%～
52.1%and8.1%～
29.4%respectively,comparedwithETgroup,(P<0.05and0.01),butAM(13-52)its
elfdoesnotpos-sestheabilitytoinhibittheVSMCs3H-TdRincor-porationandMA
PKactivation(P>0.05).Conclu┐
sionThatAMhaveaninhibitiveefectonET-1-in-ducedVSMCsproliferationandMA
PKactivation;theinhibitiveactiontakesplaceintheupstreamofMAPKactivati
oninintacelularsignalconductionpathways.Basedonthedataaswelastheprese
nceofAMspecificreceptoronVSMCs,wesuggestthatbothETandAMmaybederivedfr
omendotheliumcelstomaintainthecirculatoryfunctionalstability.
出处
《高血压杂志》
CSCD
1996年第3期171-178,共8页
Chinese Journal of Hypertension
基金
国家自然科学基金
关键词
肾上腺髓质素
内皮素
血管平滑肌细胞
高血压
adrenomedulinendothelinmito-gen-activatedproteinkinaseproliferationva
scularsmoothmusclecell
