摘要
目的探讨环氧合酶-2(COX-2)在宫颈癌中的表达。方法应用免疫组化SP法和RT-PCR 法检测宫颈癌与正常宫颈上皮组织的COX-2表达。结果免疫组化检测50例宫颈癌上皮组织的COX-2 阳性表达率为62％(31／50),而18例正常宫颈上皮COX-2阳性表达率为11．1％(2／18),有显著性差异 (P<0．05),且其与宫颈癌浸润深度和淋巴结转移的表达亦呈显著性差异(P<0．05);RT-PCR法检测25例宫颈癌COX-2mRNA表达水平为1．138±0．215,而15正常宫颈上皮为0．352±0．108,差异有显著性(P<0．05)。结论 COX-2和宫颈癌的发病密切相关。
Objective To investigate the expression of COX-2 in the cervical carcinomas and normal tis- sure. Methods Expression of COX-2 in cervical carcinomas and normal tissure was examined by immunohis- tochemical an RT-PCR method. Results The expression of COX-2 in cervical carcinoma（62%） was significantly higher than that in the normal tissue. （11.1%） （P〈0.05）and there was a positive relationship between COX-2 expression and depths of invasion, lymphnode metastages The COX-2 mRNA expression in cervical carcinoma （1.138±0.215） was significantly higher than that in normal tissue（0.352±0.108） （P〈0.05）. Conclusion There was a close relationship between COX-2 expression and cervical carcinoma.
出处
《医学新知》
CAS
2005年第4期32-34,共3页
New Medicine
