摘要
主要通过Michael加成和酰胺化逐步迭代合成反应得到聚酰胺-胺(PAMAM)树状大分子并对合成产物进行了结构表征。对生物医学有潜在应用价值的第五代(G5.0)提出了廉价易行的合成提纯步骤并通过细胞计数、MTT检测和FCM对L-929细胞系进行了体外生物相容性评价分析。结果表明,按照本试验的合成及纯化所得PAMAMG5.0对L-929细胞系在给药范围内无细胞毒性,无致瘤性,有良好的细胞相容性。
A series of polyamidoamine （PAMAM） dendrimers are synthesized with an ethylenediamine core through repetitive Michael addition and amidation, which are characterized by FT-IR, NMR, etc. The economical means and process of synthesis and purification have been presented. The cellular compatibility of Generation5.0 （ G5.0; MW = 28,788; 128amines）PAMAM has been evaluated in L-929 cells by cell counting, MTT assay and flow cytometer （FCM）. The data obtained clearly indicated our prepared PAMAM G5.0 have good cytocompatibility without cytotoxic and tumorigenicity.
出处
《中国生物医学工程学报》
EI
CAS
CSCD
北大核心
2005年第6期750-754,共5页
Chinese Journal of Biomedical Engineering
基金
北京市科技新星计划(954812000)
北京市教委科技发展计划资助(00KJ-107)
